Independent and Combined Effects of Short-term Sulforaphane Supplementation and Exercise on Immunometabolism in Healthy Adults
For patients and families
In plain language
An automatic summary of structured registry data. It is an orientation aid, not a substitute for the official protocol or a physician assessment.
- What is being studied
- The protocol lists: Sulforaphane-rich Broccoli Sprout Extract, High Intensity Interval Treadmill Running.
- Who it may be relevant to
- Registry conditions: Immunometabolism, Mitochondrial Function, Bioenergetics, Inflammation. Basic parameters: 19 years — 35 years · All.
- What needs checking
- Age, condition and sex are only basic indicators. Prior treatment, laboratory values and other mandatory requirements appear in the eligibility criteria below.
- Where it takes place
- Canada
- Next step
- Save the trial, show it to the treating physician, and confirm current recruitment with the study center. Costs, documents and travel →
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Official title
Independent and Combined Effects of Short-term Sulforaphane Supplementation and Exercise on Immunometabolism in Healthy Adults: A Randomized Crossover Study
Overview
The study consist of 3 interventional periods which will be completed by each consenting participant in a randomized, crossover fashion. The interventional periods will be separated by a minimum 1 week washout period. One of the interventions consists of 4 days of daily supplementation with the commercially available broccoli sprout extract capsules (BrocElite). Another intervention will consist of 4 days of daily supervised treadmill running. The exercise protocol consists of 4 minutes of high-intensity running running followed by a 3 minute recovery period, repeated for a total of 4 repetitions. The last intervention combines the above two and consists of 4 days of daily supplementation and daily supervised exercise training. Before and after each intervention, basic anthropometrics (weight, heart rate, and blood pressure) will be recorded, and a venous blood sample will be collected. Blood samples will be analyzed for metabolic and inflammatory markers. Participants will be asked to refrain from structured exercise, alcohol consumption, and broccoli consumption during each of the experimental periods. Before any testing, willing participants will provide informed consent, and fill out a few demographic, and lifestyle questionnaires. The study will be conducted at the University of British Columbia in Kelowna, BC, Canada.
Interventions
- Dietary supplement Sulforaphane-rich Broccoli Sprout Extract
Broc Elite Plus stabilized sulforaphane capsules from Mara Labs - Other High Intensity Interval Treadmill Running
4 cycles of: 4 minutes of intense treadmill running (85-95% HR max) followed by 3 minutes of slow, easy running.
Primary outcome measures
- Change in peripheral blood mononuclear cell oxygen consumption rate [Time frame: 0-4 days]
Secondary outcome measures (12)
- TNF-alpha secretion from LPS-stimulated whole blood [Time frame: 0-4 days]
- Change in percentage of classical monocytes before and after the 6-week intervention [Time frame: 0-4 days]
- Change in percentage of intermediate monocytes before and after the 4-day intervention [Time frame: 0-4 days]
- Change in percentage of non-classical monocytes before and after the 4-day intervention [Time frame: 0-4 days]
- Change in TLR-4 expression on classical monocytes before and after the 4-day intervention [Time frame: 0-4 days]
- Change in TLR-4 expression on intermediate monocytes before and after the 4-day intervention [Time frame: 0-4 days]
- Change in TLR-4 expression on non-classical monocytes before and after the 4-day intervention [Time frame: 0-4 days]
- Change in white blood cell counts before and after the 4-day intervention [Time frame: 0-4 days]
- TNF-alpha secretion from PolyIC-stimulated whole blood [Time frame: 0-4 days]
- Change in peripheral blood mononuclear cell extracellular acidification rate before and after the 4-day period [Time frame: 0-4 days]
- Change in PBMC mitotracker green staining before and after the 4-day period [Time frame: 0-4 days]
- Change in PBMC mitoSOX red staining before and after the 4-day period [Time frame: 0-4 days]
Eligibility criteria
Inclusion criteria
- aged between 19 and 35 years
- not a current smoker
- physically active based on the Canadian Physical Activity Guidelines (150 minutes of weekly physical activity)
Exclusion criteria
- a history of cardiometabolic diseases or respiratory diseases (e.g., chronic obstructive pulmonary disease, asthma, diabetes, coronary artery disease)
- currently following a ketogenic diet
- body mass index (BMI) over 30 kg/m2
- unable to read or communicate in English
- having received a vaccination or experienced an upper respiratory tract infection in the last 4 weeks
- having donated more than 0.5 L of blood within the last 4 weeks
- currently pregnant
- have contraindications to high intensity exercise (assessed using the CSEP Get Active Questionnaire)
Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.
Healthy volunteers: Yes
Study design
- Allocation
- Randomized
- Model
- Crossover
- Masking
- Open label
- Primary purpose
- Basic science
Study locations
Canada · 1 center
- Reichwald Health Sciences Centre — Kelowna
Identifiers
NCT: NCT07668596 · H25-03273