Circulating and Urine Tumor DNA Dynamics Predict Minimal Residual Disease and Recurrence Risk in Locally Advanced Upper Tract Urothelial Carcinoma
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In plain language
An automatic summary of structured registry data. It is an orientation aid, not a substitute for the official protocol or a physician assessment.
- What is being studied
- The protocol lists: Adjuvant chemotherapy, adjuvant immunotherapy.
- Who it may be relevant to
- Registry conditions: Muscle Invasive Upper Tract Urothelial Carcinoma. Basic parameters: from 18 years · All.
- What needs checking
- Age, condition and sex are only basic indicators. Prior treatment, laboratory values and other mandatory requirements appear in the eligibility criteria below.
- Where it takes place
- China
- Next step
- Save the trial, show it to the treating physician, and confirm current recruitment with the study center. Costs, documents and travel →
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Official title
Circulating and Urine Tumor DNA Dynamics Predict Minimal Residual Disease and Recurrence Risk in Locally Advanced Upper Tract Urothelial Carcinoma(CURATE-UTUC): A Multicenter Prospective Longitudinal Cohort Study
Overview
In our study, the ultra-deep sequencing of circulating tumor DNA (ctDNA) and urine tumor DNA (utDNA) were performed to assess whether ctDNA and utDNA can be used as predictive biomarkers for the detection of minimal residual disease (MRD) and early diagnosis of UTUC recurrence, and explored the role of ctDNA and utDNA detection of MRD in the prediction of adjuvant therapy efficacy and prognostic evaluation.
Detailed description
60% of Upper Urinary Tract Urothelial Carcinoma (UTUC) patients with muscle-invasive disease at diagnosis, which progresses rapidly, aggressively, and has a poor prognosis. Up to 30-40% of the patients may develop bladder recurrance after radical nephroureterectomy for primary upper tract urothelial carcinoma.
Minimal residual disease (MRD) refers to the small number of malignant cells that remain after curative treatments (curative intent surgical resection, radiotherapy, and/or chemotherapy). MRD is common in patients with blood cancer, and is known to be associated with recurrence and poor prognosis. Recent studies also reported that MRD-negative in postoperative solid tumors such as colorectal/colon cancer and muscle-invasive bladder cancer is associated with better survival outcomes. However, the clinical values of MRD monitoring for adjuvant therapy in postoperative UTUC remain inadequate.
A total of 84 patients with stage II-IV UTUC will be recruited in this clinical trial. The following plasma samples, urine samples and tumor tissues will be collected from each patient including T0 (preoperatively, 2\[1-3\] days before surgery), T1 (28±3 days postoperatively), T2 (within 7 days after cycle 2 adjuvant therapy), T3 (end-of-treatment), and quarterly/semi-annually during surveillance (T4-T6) until recurrence/24 months. In addition, demographic and tumor characteristics of the patients will be collected for subsequent analysis, including age, sex, tumor stage, pathological stage, disease couse time, etc. Tumor tissues and matched peripheral blood were collected before treatment and WES was used ctDNA detection techniques. For each patient, we selected up to 40 clonal somatic mutations for personalized, tumor informed ctDNA assay design.Statistical analyses will be performed to analyze the survival outcomes and to explore the clinical value of MRD monitoring for adjuvant therapy in postoperative UTUC.
Interventions
- Drug Adjuvant chemotherapy
cisplatin/carboplatin-gemcitabine 4-6 cycles - Drug adjuvant immunotherapy
immunotherapy for one year
Primary outcome measures
- extravesical recurrence free survival [Time frame: 2 year]
Secondary outcome measures (5)
- Lead time of molecular recurrence detection [Time frame: 2 year]
- intravesical recurrence free survival [Time frame: 2 year]
- recurrence-free survival [Time frame: 2 year]
- Overall survival [Time frame: 2 year]
- Diagnostic performance of MRD assay [Time frame: 2 year]
Eligibility criteria
Inclusion criteria
- pathological comfirmed T2-4 or N+ and M0 upper tract urothelial carcinoma
- Male or female aged ≥18 years old who are willing to sign the informed consent form
- have no distant metastasis
- have an ECOG 0 to 2
- upper tract urothelial carcinoma patients received radical nephroureterectomy
- have no multiple primary carcinoma
- received adjuvant chemotherapy or immunotherapy after surgery within 12 weeks
- ≥2 postoperative liquid biopsy assessments (T1 and T2)
Exclusion criteria
- a prior history of bladder or synchronous bladder cancer
- Pregnant or lactating women, or patients who are fertile but do not take contraceptive measures;
- Severe infection;
- Severe heart disease;
- Uncontrollable neurological or mental disorders;
- Severe diabetes mellitus;
- Patients with severe autoimmune diseases.
- Neoadjuvant therapy exposure
- No bilateral UTUC
- Surveillance time < month
- <2 postoperative MRD surveillance assessments
Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.
Healthy volunteers: No
Study design
- Observational model
- Cohort
Study locations
China · 1 center
- Renji Hospital — Shanghai
Identifiers
NCT: NCT05595408 · CURATE-UTUC