TCP-600 Supplementation and Performance Response in Professional Football Players

This is a randomized, double-blind, placebo-controlled validation study conducted in professional male football players. The study is designed to externally validate and extend individual responder profiles previously derived from randomized controlled trials investigating taurine-, caffeine-, and phosphatidylserine-containing supplementation in professional football players.

Participants in the validation cohort will be randomly assigned to receive either placebo or a phosphatidylserine-containing multi-ingredient supplement containing taurine, caffeine, and phosphatidylserine. Phosphatidylserine will be provided at a dose of 600 mg per day. The supplementation period will last 28 days. The placebo will be matched as closely as possible in appearance, number of units, packaging, and administration schedule.

The main aim of the study is to determine whether baseline characteristics can help identify players who are more likely to respond positively to the supplementation protocol. The multidimensional responder profile will include predefined domains related to physical performance and fatigue resistance, cognitive and mental fatigue-related outcomes, recovery/readiness-related outcomes, and selected football-specific perceptual-cognitive performance.

Before and after the supplementation period, participants will complete non-invasive assessments. These may include 30 m sprint performance, simple or choice reaction time, computerized cognitive tasks, mental fatigue ratings, heart rate variability indices such as LnRMSSD, wellness/readiness questionnaires, sleep quality, perceived fatigue, and selected football-specific performance variables where available.

The perceptual-cognitive domain may include football-specific decision-making accuracy, response time, tactical inhibitory decision-making under cognitive load, situational awareness, visual search-related variables, and selected field-transfer outcomes where available. These measures will be used to explore whether players who respond favorably in physical or recovery-related domains also show favorable responses in cognitive fatigue resistance, decision-making, or perceptual-cognitive football performance.

No blood, saliva, urine, genetic material, or other biological samples will be collected. All assessments will be non-invasive. The validation cohort will test whether responder profiles developed from previous trials can be applied to an independent group of professional football players. The study may support a more individualized approach to performance nutrition by identifying whether baseline autonomic, cognitive, fatigue-related, recovery-related, or perceptual-cognitive characteristics are associated with a greater likelihood of response to phosphatidylserine-containing multi-ingredient supplementation.

• Change in 30 m Sprint Time [Срок оценки: Baseline to post-intervention after 28 days of supplementation]
• Change in Cognitive Fatigue Task Reaction Time [Срок оценки: Baseline to post-intervention after 28 days of supplementation]
• Change in Perceived Mental Fatigue VAS Score [Срок оценки: Baseline to post-intervention after 28 days of supplementation.]
• Change in Final 3-Day Mean LnRMSSD [Срок оценки: Baseline monitoring period to final 3 days of supplementation.]
• Change in Readiness-to-Train Rating Scale Score [Срок оценки: Baseline monitoring period to final 3 days of supplementation.]
• Change in Hooper and Mackinnon Wellness Questionnaire Fatigue Item Score [Срок оценки: Baseline monitoring period to final 3 days of supplementation.]
• Area Under the Receiver Operating Characteristic Curve for the Predefined Responder Model [Срок оценки: Post-intervention after 28 days of supplementation.]
• Number of Participants With Supplement-Related Adverse Events [Срок оценки: During the 28-day supplementation period]
• Change in Football-Specific Video Decision-Making Task Response Time [Срок оценки: Baseline to post-intervention after 28 days of supplementation.]
• Change in Football-Specific Video Decision-Making Task Accuracy [Срок оценки: Baseline to post-intervention after 28 days of supplementation.]
• Change in Eye-Tracking Time to First Fixation on the Optimal Tactical Option [Срок оценки: Baseline to post-intervention after 28 days of supplementation.]