Effects of Estrogen on Muscle Gain During 12-weeks of Exercise in Post-menopausal Women

Обзор

As females age and transition through menopause, the decline in oestrogen level profoundly affects skeletal muscle mass and function. HER-MUSCLE aims to unravel the mechanisms by which oestrogen enhances muscle growth, providing insights for targeted therapies to improve the health and physical function of postmenopausal females. Focusing on postmenopausal females, an increasingly at-risk demographic, HER-MUSCLE addresses a critical gap in understanding how oestrogen influences muscle mass and function during anabolic (exercise) conditions. The project involves: 1. Clinical Trial: Postmenopausal females will receive either oestrogen or placebo, twelve weeks of exercise training to detect oestrogen regulatory role on muscle mass and function. 2. Molecular Analysis: Advanced techniques will study the muscle microenvironment, focusing on muscle stem cells (MuSCs), fibro-adipogenic progenitors (FAPs), and other cells critical for muscle regeneration and maintenance. 3. Mitochondrial Function assessed in vivo via magnetic resonance spectroscopy: The impact of oestrogen on mitochondrial health will be examined, exploring how it preserves mitochondrial function and ability to recovery and resist fatigue in response to muscle contractions. Our preliminary data indicate that oestrogen can promote muscle protein synthesis. HER-MUSCLE aims to pave the way for novel therapeutic strategies to manage sarcopenia in postmenopausal women, ultimately leading to better health outcomes and enhanced well-being for this growing population segment.

Подробное описание

As females age and transition through menopause, the decline in oestrogen level profoundly affects skeletal muscle mass and function. HER-MUSCLE aims to unravel the mechanisms by which oestrogen enhances muscle growth, providing insights for targeted therapies to improve the health and physical function of postmenopausal females.

Focusing on postmenopausal females, an increasingly at-risk demographic, HER-MUSCLE addresses a critical gap in understanding how oestrogen influences muscle mass and function during anabolic (exercise) conditions.

The project involves:

1. Clinical Trial: Postmenopausal females will receive either oestrogen or placebo, twelve weeks of exercise training to detect oestrogen regulatory role on muscle mass and function. 2. Molecular Analysis: Advanced techniques will study the muscle microenvironment, focusing on muscle stem cells (MuSCs), fibro-adipogenic progenitors (FAPs), and other cells critical for muscle regeneration and maintenance. 3. Mitochondrial Function assessed in vivo via magnetic resonance spectroscopy: The impact of oestrogen on mitochondrial health will be examined, exploring how it preserves mitochondrial function and ability to recovery and resist fatigue in response to muscle contractions.

Our preliminary data indicate that oestrogen can promote muscle protein synthesis. HER-MUSCLE aims to pave the way for novel therapeutic strategies to manage sarcopenia in postmenopausal women, ultimately leading to better health outcomes and enhanced well-being for this growing population segment.

Вмешательства

• Препарат Estrogel
  The other group receive placebo treatment
• Поведенческое Physical exercise
  Both groups will go through 12 weeks of supervised physical exercise, consisting of both strength training and cardiovascular training

Первичные конечные точки

• Muscle mass [Срок оценки: 12 weeks]

Вторичные конечные точки (12)

• Mitochondria Function in vivo measured as phosphocreatine recovery rate [Срок оценки: 12 weeks]
• Mitochondria Function in vitro measured as maximal oxygen consumption [Срок оценки: 12 weeks]
• Body composition [Срок оценки: 12 weeks]
• Satelitte cells [Срок оценки: 12 weeks]
• Muscle fiber cross-sectional Area [Срок оценки: 12 weeks]
• Expression of Muscle proteins [Срок оценки: 12 weeks]
• FACS Analysis [Срок оценки: 12 weeks]
• Muscle Strength [Срок оценки: 12 weeks]
• Functional test [Срок оценки: 12 weeks]
• Cardiovascular fitness [Срок оценки: 12 weeks]
• Maximal fat oxidation rate [Срок оценки: 12 weeks]
• Resisting metabolic rate [Срок оценки: 12 weeks]

Критерии участия

Критерии включения

• 1-10 years since last menstrual bleeding
• Age > 40 years old
• BMI 20-30

Критерии исключения

• Follicular stimulating hormone < 30 mmol/L
• Systematic strength training during the last year (> 1 strength training session per week)
• Injuries to the legs which may prevent participation in the physical training program
• Magnetizable metals or electrical devices implanted in the body, such as a pacemaker
• Use of medication that can influence the effect of immobilization and/or training
• Muscular or joint disorders which may affect the results
• Metabolic diseases (such as diabetes and cardiovascular diseases)
• Previous or present liver or cancer disease
• Current or previous thrombosis
• Porphyria
• Epilepsia
• Systemic autoimmune disease
• Edema
• Smoking or use of other nicotine containing products
• Claustrophobia
• Addictive behavior, defined as abuse of cannabis, opioids, or other intoxicating substances.
• Lack of ability to cooperate
• Blood parameters out of normal range at the health check
• Blood pressure above 140/90 mmHg

Критерии приведены из реестра в оригинале (на английском). Окончательную оценку соответствия проводит исследовательский центр.

Здоровые добровольцы: Да

Дизайн исследования

Распределение

Рандомизированное

Модель

Параллельные группы

Маскирование

Тройное слепое

Основная цель

Фундаментальное исследование

Центры проведения

Дания · 1 центр

• Aarhus University — Aarhus

Идентификаторы

NCT: NCT07617454 · E30 HER-MUSCLE Intervention

Первоисточники (государственные реестры)

• ClinicalTrials.gov (NIH, США)

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