Glycocalyx and Oxidative Stress in Endothelial Function (Part 1)

Официальное название

The Role of Glycocalyx and Oxidative Stress in Endothelial Function (Part 1)

Обзор

The primary research objective of the project is to determine the role of eGC in microvascular reactivity in CKD (cross-sectional study 1). The main objective of the project is to increase the excellence and interdisciplinarity of scientific work at MEFOS by connecting researchers from different scientific fields, improving conditions and resources for scientific work, and increasing international cooperation, in line with strategic objective 1: raising scientific excellence. The results of the project could provide pathophysiological insight into the development and maintenance of endothelial dysfunction in CKD, as well as identify new biomarkers for CKD.

Подробное описание

The primary objective of this project is to determine the role of endothelial glycocalyx (eGC) in impaired microvascular endothelium-dependent reactivity in patients with chronic kidney disease compared to healthy and hypertensive patients without complications (cross-sectional study 1).

Specific objective 1.1: Measure the reactivity of the peripheral microcirculation (using a laser Doppler blood flow meter) and the thickness of eGC (using Glycocheck) in the specified groups.

Specific objective 1.2: Quantify soluble markers of eGC, oxidative stress, and inflammation from serum samples and peripheral blood mononuclear cells (PBMC), as well as antioxidant capacity in the specified groups.

Specific objective 1.3: Determine the relationship between the results of functional tests, eGC, and markers from specific objective 1.2.

Первичные конечные точки

• Microvascular reactivity [Срок оценки: Baseline, at one time point (first visit to the laboratory)]
• Endothelial glycocalyx [Срок оценки: Baseline, at one time point (first visit to the laboratory)]

Вторичные конечные точки (4)

• Endothelial glycocalyx soluble markers [Срок оценки: Baseline, at one time point (first visit to the laboratory)]
• Concentration of MPO, 8-iso prostaglandin F2a, OxLDL, AOPP and intracellular oxidative stress production [Срок оценки: Baseline, at one time point (first visit to the laboratory)]
• Macrophage frequency [Срок оценки: Baseline, at one time point (first visit to the laboratory)]
• Antioxidant enzyme (SOD, CAT, GPx) concentration and activity [Срок оценки: Baseline, at one time point (first visit to the laboratory)]

Критерии участия

Критерии включения

• patients with CKD of hypertensive etiology (grades 1-2 and 3-5; eGFR CKD-EPI GFR ≥ 90 ml/min/1.73 m², i.e. < 60 mL/min/1.73 m2), non-diabetics, non-smokers
• patients with hypertension (according to ESH2023 Guidelines) without kidney damage (eGFR CKD-EPI >90 mL/min/1.73 m2) non-diabetics, non-smokers
• healthy volunteer subjects as controls

Критерии исключения

• kidney disease of any other etiology except hypertension
• endocrine, autoimmune or inflammatory diseases that could affect kidney function.
• smoking
• diabetes

Критерии приведены из реестра в оригинале (на английском). Окончательную оценку соответствия проводит исследовательский центр.

Здоровые добровольцы: Да

Дизайн исследования

Модель наблюдения

Случай-контроль

Центры проведения

Хорватия · 1 центр

• Osijek University Hospital — Osijek

Идентификаторы

NCT: NCT07437729 · 602-04/25-08/07

Первоисточники (государственные реестры)

• ClinicalTrials.gov (NIH, США)

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