Идёт набор NCT07345455

Probiotics on Sperm Quality in Male Infertility Patients

Без фазы С лечением Male Infertility Oligozoospermia Asthenozoospermia Teratozoospermia

Ориентир для пациента и семьи

Простыми словами

Автоматическая сводка по структурированным данным реестра. Она помогает сориентироваться, но не заменяет официальный протокол или оценку врача.

Что изучают: В протоколе указаны: Probiotics, Placebo.
Кому может быть актуально: Состояния в реестре: Male Infertility, Oligozoospermia, Asthenozoospermia, Teratozoospermia. Базовые параметры: 20 лет — 45 лет · Мужчины.
Что важно проверить: Возраст, диагноз и пол — только базовые ориентиры. Предыдущее лечение, анализы и другие обязательные условия указаны ниже в критериях участия.
Где проводится: Тайвань
Следующий шаг: Сохраните исследование, покажите его лечащему врачу и уточните актуальный статус у исследовательского центра. Расходы, документы и поездка →

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Официальное название

Effect of Probiotics on Sperm Quality in Male Infertility Patients

Обзор

This randomized, placebo-controlled pilot study aims to evaluate the effects of probiotic supplementation on sperm quality in male patients diagnosed with infertility. Male infertility accounts for approximately 40% of all infertility cases and is closely related to abnormalities in sperm count, motility, and morphology. Factors such as oxidative stress, inflammation, and DNA fragmentation are known to impact sperm function and subsequent fertilization potential negatively. Probiotics are microorganisms that confer health benefits by improving the intestinal microenvironment and regulating immunity. Emerging research suggests that probiotics may reduce oxidative stress and DNA fragmentation in men with asthenozoospermia; however, clinical data on human sperm remains limited. This study seeks to determine whether specific probiotic strains can improve sperm parameters and function in patients with unexplained oligozoospermia, asthenozoospermia, or oligoasthenoteratozoospermia. The study will enroll 60 male participants aged 20 to 45 who meet specific inclusion criteria, including a sperm concentration of less than 50 million/mL, motility less than 40%, and normal morphology (Kruger) less than 4%. Participants will be randomly assigned to either an experimental group receiving probiotics or a control group receiving a placebo for approximately 100 days. Semen samples will be collected and analyzed at three time points: before the intervention (Day 0), during the intervention (Day 60), and at the end of the study (Day 100). The primary objective is to assess changes in sperm concentration, motility, and morphology using the Computer-Aided Sperm Analysis (CASA) system. Secondary objectives include evaluating sperm DNA integrity using the Sperm Chromatin Structure Assay (SCSA) by flow cytometry and assessing sperm function via the acrosome reaction assay. The results of this pilot study will help determine the potential therapeutic role of probiotics in the management of male infertility.

Подробное описание

Content: Male infertility accounts for approximately 40% of infertility cases and is often characterized by abnormalities in sperm count, motility, and morphology. Factors such as oxidative stress, environmental hormones, and inflammation can lead to sperm DNA fragmentation and mitochondrial dysfunction, ultimately affecting fertilization potential. While probiotics are known to improve gut microbiome balance and regulate immunity, limited studies suggest they may also improve sperm motility and reduce DNA fragmentation in men with asthenozoospermia.

This pilot study employs a randomized, placebo-controlled design to evaluate the efficacy of probiotic supplementation in improving sperm quality. A total of 60 male participants with unexplained infertility (specifically oligozoospermia, asthenozoospermia, or oligoasthenoteratozoospermia) will be recruited. Participants will be divided into two groups: an experimental group receiving probiotics and a control group receiving a placebo.

The study duration for each participant is approximately 100 days, with semen samples collected at three distinct time points:

1. Day 0 (Start Test): Baseline sample collection. 2. Day 60 (Mid Test): Interim sample collection. 3. Day 100 (Final Test): Post-intervention sample collection.

Semen analysis will be conducted using the following methodologies:

* Sperm Concentration and Motility: Analyzed using a Computer-Aided Sperm Assay (CASA) to measure parameters including total motility, progressive motility, VAP, VCL, VSL, Straightness, and Linearity. * Sperm DNA Integrity: Assessed via the Sperm Chromatin Structure Assay (SCSA) using flow cytometry. Sperm are treated with acridine orange; red fluorescence indicates single-stranded DNA (fragmentation), while green fluorescence indicates double-stranded DNA. Results are expressed as the DNA Fragmentation Index (DFI).

Sperm Function (Acrosome Reaction): Evaluated using Peanut Agglutinin (PNA) staining. The assay determines the percentage of sperm capable of undergoing the acrosome reaction, a necessary step for fertilization.

Inclusion Criteria

* Gender: Male. * Age: 20 to 45 years old. * Diagnosis: Diagnosed with unexplained oligozoospermia, asthenozoospermia, or oligoasthenoteratozoospermia. * Semen Parameters:

* Sperm concentration \< 5×106/mL (Note: Source indicates "5x10/mL", contextually adjusted to standard unit for oligozoospermia). * Sperm motility \< 40%. * Sperm morphology (Kruger strict criteria) \< 4%. Exclusion Criteria

1. History of hormonal disorders or epididymo-orchitis. 2. Substance abuse, including drugs or excessive alcohol consumption. 3. Diabetes mellitus. 4. Kidney disease (defined as a doubling of creatinine levels or more). 5. Chronic liver disease. 6. Varicocele. 7. Current use of medications that interfere with hormones. 8. Occupational or environmental exposure to pesticides, heavy metals, or solvents. 9. Intake of antioxidant supplements within the past three months. 10. Body Mass Index (BMI) of 30 kg/m² or higher.

Вмешательства

Пищевая добавка Probiotics
The subjects take 2 probiotic capsules after each meal daily.
Пищевая добавка Placebo
The control group takes two placebo capsules (containing no probiotics) after meals daily.

Первичные конечные точки

Change From Baseline in Sperm Concentration Assessed by Computer-Aided Sperm Analysis (CASA) [Срок оценки: Day 60, Day 100]
Change From Baseline in Total Sperm Motility Percentage Assessed by Computer-Aided Sperm Analysis (CASA) [Срок оценки: Day 60, Day 100]
Change From Baseline in Percentage of Normal Sperm Morphology Assessed by Computer-Aided Sperm Analysis (CASA) [Срок оценки: Day 60, Day 100]

Вторичные конечные точки (2)

Change From Baseline in Sperm DNA Fragmentation Index (DFI) Assessed by SCSA [Срок оценки: Day 60, Day 100]
Change From Baseline in Sperm Acrosome Reaction Rate Assessed by PNA Staining [Срок оценки: Day 60, Day 100]

Критерии участия

Критерии включения

Males aged 20 to 45 years
Diagnosis of unexplained oligozoospermia, asthenozoospermia, or oligoasthenoteratozoospermia
Sperm concentration < 5 x 10\^6/mL
Sperm total motility < 40%
Sperm morphology (Kruger strict criteria) < 4%