Spatiotemporal Single-cell Atlas of Peri-implant Soft Tissue Healing
Ориентир для пациента и семьи
Простыми словами
Автоматическая сводка по структурированным данным реестра. Она помогает сориентироваться, но не заменяет официальный протокол или оценку врача.
- Что изучают
- В протоколе указаны: Healing Abutment Gingival Healing Post-Implantation.
- Кому может быть актуально
- Состояния в реестре: Peri-implant Soft Tissue Healing. Базовые параметры: 18 лет — 65 лет · Все.
- Что важно проверить
- Возраст, диагноз и пол — только базовые ориентиры. Предыдущее лечение, анализы и другие обязательные условия указаны ниже в критериях участия.
- Где проводится
- Китай
- Следующий шаг
- Сохраните исследование, покажите его лечащему врачу и уточните актуальный статус у исследовательского центра. Расходы, документы и поездка →
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Обзор
This study aims to explore the dynamic changes in different cell types and their molecular regulatory mechanisms during the healing process of peri-implant soft tissues, using single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST). The research will focus specifically on the healing of soft tissues around dental implants in human subjects with the use of a healing abutment. Participants in the healing abutment group will have a micro healing abutment placed immediately after implant placement. Tissue samples will be collected at various time points (Day 4, Day 14, and Day 70) to construct a detailed single-cell map of the healing process. Spatial transcriptomics will be integrated to preserve tissue architecture, enabling the identification of the spatial distribution of different cell types and their interactions within the peri-implant microenvironment. This combined approach will allow for a comprehensive characterization of cellular interactions, spatial gene expression patterns, and regulatory networks specific to healing abutments. The goal is to identify key regulatory factors that could improve peri-implant soft tissue healing and help prevent peri-implantitis.
Вмешательства
- Процедура Healing Abutment Gingival Healing Post-Implantation
The healing process of peri-implant soft tissues will be studied using a micro healing abutment (2 mm), which will be placed at least three months after implant Stage I surgery. Gingival tissue samples will then be collected at Day 4, Week 2, Week 4, and Week 8-10. At each time point, a circular incision around the micro abutment will be made using a circular punch, and the gingival tissue together with the abutment will be retrieved under local anesthesia. After sampling, the micro abutment wil
Первичные конечные точки
- Primary Outcome 1: Differentially Expressed Genes (DEGs) in Peri-implant Soft Tissues [Срок оценки: Day 4, Week 2, Week 4 and Week 8-10 post-implantation.]
- Cell Type Composition in Peri-implant Soft Tissues [Срок оценки: Day 4, Week 2, Week 4, and Week 8-10 post-implantation.]
- Enriched Biological Pathways [Срок оценки: Day 4, Week 2, Week 4, and Week 8-10 post-implantation]
- Ligand-Receptor Interaction Strength [Срок оценки: Day 4, Week 2, Week 4, and Week 8-10 post-implantation]
- Spatial Gene Expression Patterns [Срок оценки: Day 4, Week 2, Week 4 and Week 8-10 post-implantation.]
Вторичные конечные точки (4)
- Histological Tissue Morphology [Срок оценки: Day 4, Week 2, Week 4 and Week 8-10 post-implantation.]
- Immune and Stromal Cell Quantification [Срок оценки: Day 4, Week 2, Week 4 and Week 8-10 post-implantation.]
- Junctional Epithelium Length [Срок оценки: Day 4, Week 2, Week 4, and Week 8-10 post-implantation.]
- Basement Membrane Protein Expression [Срок оценки: Day 4, Week 2, Week 4, and Week 8-10 post-implantation.]
Критерии участия
Критерии включения
- Age: 18-65 years
- Healthy individuals without chronic medical conditions or severe systemic diseases
- Able to comply with study requirements (e.g., follow-up visits)
- No history of periodontal disease or active infection
- Already completed four or more implant placements in the posterior tooth region
- Adequate gingival thickness to allow for sampling and proper healing
Критерии исключения
- Individuals with systemic conditions that affect wound healing (e.g., diabetes, immunosuppressive therapy)
- Smoking or alcohol abuse
- Pregnant or breastfeeding women
- History of severe allergic reactions to implant materials
- Participation in another clinical study at the same time
Критерии приведены из реестра в оригинале (на английском). Окончательную оценку соответствия проводит исследовательский центр.
Здоровые добровольцы: Да
Дизайн исследования
- Распределение
- Не применимо
- Модель
- Одна группа
- Маскирование
- Открытое
- Основная цель
- Фундаментальное исследование
Центры проведения
Китай · 1 центр
- School and Hospital of Stomatology, Fujian Medical University — Фучжоу
Идентификаторы
NCT: NCT07279090 · 20250829