Omega-3 Fatty Acids in Muscle Function and Health

Официальное название

Effects of Omega-3 Fatty Acid Supplementation on Muscle Performance, Neuromuscular Function, and Inflammation at Rest and Following Eccentric Exercise.

Обзор

This study aims to investigate the effects of omega-3 fatty acid supplementation on muscle performance, neuromuscular function, inflammation, and oxidative stress at rest and after eccentric exercise. The findings may provide insights into the role of omega-3 fatty acids in muscle function, physiology, and metabolism following muscle-damaging exercise. Additionally, the effects of omega-3 fatty acids on cardiovascular health will be investigated.

Подробное описание

The study is a double-blind, randomized controlled trial. Two parallel groups of 16 healthy males and females each (sex counterbalanced, 20-45 years) will randomly take 3 g of fish oil \[1500 mg EPA (eicosapentaenoic acid) + 750 mg DHA (docosahexaenoic acid), Now Foods, USA\] or a placebo (3 g of olive oil) for 12 weeks. Before taking the supplement, participants will perform a unilateral isokinetic eccentric exercise session of maximal voluntary intensity (10 repetitions x 10 sets of knee extensions at a speed of 60 degrees per second). Dominant and non-dominant legs will be allocated equally and randomly across participants. Physiological assessments and blood samples will be collected immediately before exercise and 2 and 4 days after eccentric exercise (bout 1). Then, participants will start the supplementation for 12 weeks. At the end of the supplementation period, participants will perform the same eccentric exercise protocol (bout 2) with their opposite leg. The same assessments and blood sampling will be conducted at the same time points (i.e., immediately before and 2 and 4 days after eccentric exercise).

Вмешательства

• Пищевая добавка Omega-3
  Participants will take three capsules per day (a total of 1,500 mg EPA and 750 mg DHA). They will take 1 g with each main meal (breakfast, lunch, and dinner).
• Пищевая добавка Placebo comparator
  Participants will take 3 g of olive oil. They will take 1 g with each main meal (breakfast, lunch, and dinner).

Первичные конечные точки

• Muscle Strength [Срок оценки: Changes in muscle strength after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Maximal Oxygen Uptake (VO₂ max) [Срок оценки: Changes in VO₂ max after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Muscle Fatigue Index (MFI) [Срок оценки: Changes in MFI after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Muscle Oxygenation [Срок оценки: Changes in muscle oxygenation after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Creatine Kinase [Срок оценки: Change in creatine kinase activity after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Range of Motion (ROM) [Срок оценки: Change in ROM after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Muscle Thickness [Срок оценки: Change in muscle thickness after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Echo Intensity [Срок оценки: Change in echo intensity after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Blood Lipids Profile [Срок оценки: Change in blood lipids from baseline (immediately before supplementation) to 12 weeks after the supplementation period.]

Вторичные конечные точки (12)

• Omega-3 Index [Срок оценки: Change in omega-3 index in red blood cells from baseline (immediately before supplementation) to 12 weeks after the supplementation period.]
• Rate of Force Development (RFD). [Срок оценки: Change in RFD after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Electromechanical Delay (EMD) [Срок оценки: Change in EMD after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Force Matching Error (FME) [Срок оценки: Change in FME after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Joint Position Sense (JPS) [Срок оценки: Change in JPS after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Delayed Onset Muscle Soreness (DOMS). [Срок оценки: Change in DOMS after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Countermovement Jump (CMJ) [Срок оценки: Change in CMJ after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Contact Time [Срок оценки: Change in contact time after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Stability Index [Срок оценки: Change in stability index after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• F2-Isoprostanes [Срок оценки: Change in F2-Isoprostanes after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Glutathione [Срок оценки: Change in glutathione after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]
• Protein carbonyls [Срок оценки: Change in protein carbonyls after the first bout (week 0) and the second bout of exercise (week 12) from baseline (immediately before each bout) and at day 2 and day 4 after each exercise bout.]

Критерии участия

Критерии включения

• Body Mass Index (BMI) within the normal range (18.5-25 kg/m²).
• Healthy individuals without severe medical conditions (e.g., diabetes, coronary artery disease, hypertension, neuromuscular disorders).
• No musculoskeletal injury in the lower limbs (e.g., bone fracture, tendon rupture, grade III muscle strain) within the last 6 months before enrollment.
• Negative responses to all questions on the PAR-Q questionnaire indicating no contraindications to participating in exercise testing.
• Mentally capable of providing informed consent.

Критерии исключения

• Use of any pharmaceutical medications (e.g., statins, aspirin, ibuprofen).
• Use of dietary supplements (e.g., protein, vitamins) within the last 3 months before enrollment.
• Participation in a structured training program involving more than three training sessions per week during the study period.
• Following a special diet (e.g., intermittent fasting, ketogenic diet, vegetarian diet).
• Allergy or intolerance to fish oil or olive oil.

Критерии приведены из реестра в оригинале (на английском). Окончательную оценку соответствия проводит исследовательский центр.

Здоровые добровольцы: Да

Дизайн исследования

Распределение

Рандомизированное

Модель

Параллельные группы

Маскирование

Четверное слепое

Основная цель

Поддерживающая терапия

Центры проведения

Cyprus · 1 центр

• European University Cyprus — Nicosia

Идентификаторы

NCT: NCT07134140 · EUC omega-3

Первоисточники (государственные реестры)

• ClinicalTrials.gov (NIH, США)

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