Effect of Immediate Skin-to-Skin Contact on Neonatal Heart Rate Variability After Cesarean Secton

At the time of birth, both the newborn and the mother undergo a complex and dynamic process of physiological and psychological adaptation, often referred to as perinatal adaptive stress. This adaptive stress response involves significant alterations in neuroendocrine and autonomic function, including activation of the hypothalamic-pituitary-adrenal (HPA) axis and fluctuations in autonomic nervous system (ANS) activity. These physiological responses can be objectively assessed using various biomarkers and physiological parameters. Among the most informative and non-invasive indicators of autonomic regulation is heart rate variability (HRV)-a measure of the variation in time intervals between consecutive heartbeats (RR intervals), which reflects the interplay between the sympathetic and parasympathetic branches of the ANS.

HRV has been widely used in neonatal and maternal research as a surrogate marker of stress, resilience, and autonomic function. High HRV is generally associated with enhanced parasympathetic (vagal) tone and a more flexible and adaptive physiological state, whereas low HRV is indicative of stress, reduced vagal activity, and sympathetic predominance. The immediate postpartum period, particularly following cesarean delivery, represents a critical window during which both maternal and neonatal physiological systems transition from intrauterine to extrauterine life, often in the absence of the hormonal and mechanical stimuli associated with vaginal delivery.

The proposed randomized controlled trial aims to investigate the effect of early skin-to-skin contact (SSC) between mother and newborn on HRV in both participants following planned cesarean section. Early SSC has been shown in previous studies to promote maternal-infant bonding, thermoregulation, breastfeeding initiation, and physiological stability; however, its impact on autonomic modulation as assessed by HRV-particularly in cesarean births-remains underexplored.

The central hypothesis of this study is that immediate and sustained skin-to-skin contact will result in significantly greater HRV in both neonates and mothers, as compared to a control group receiving standard post-cesarean care. This increase in HRV is expected to reflect reduced perinatal stress, enhanced vagal activation, and improved autonomic balance during the early postpartum period.

The study will enroll 80 mother-newborn dyads with a gestational age of ≥39 weeks undergoing elective cesarean section. Participants will be randomly assigned to either the intervention group, in which uninterrupted skin-to-skin contact will be initiated immediately and maintained for at least 15 minutes following birth, or the control group, which will receive routine postnatal care without early SSC. Each group will comprise 40 dyads.

Electrocardiographic (ECG) monitoring will be conducted in both mothers and newborns during the first 15 minutes following birth to capture real-time cardiac data. In addition, neonatal ECG will be monitored at 6, 12 and 24 hours postpartum. HRV will be analyzed using time-domain methods, in accordance with established international standards.

• Maternal RMSSD 15 minutes after birth [Срок оценки: 15 minutes following cesarean birth]
• Maternal SDNN 15 minutes after birth [Срок оценки: 15 minutes following cesarean birth]
• Maternal pNN50 15 minutes after birth [Срок оценки: 15 minutes following cesarean birth]
• Neonatal RMSSD 6 hours after birth [Срок оценки: 6 hours after cesarean birth]
• Neonatal SDNN 6 hours after birth [Срок оценки: 6 hours after cesarean birth]
• Neonatal pNN50 6 hours after birth [Срок оценки: 6 hours after cesarean birth]
• Neonatal RMSSD 12 hours after birth [Срок оценки: 12 hours after cesarean birth]
• Neonatal SDNN 12 hours after birth [Срок оценки: 12 hours after cesarean birth]
• Neonatal pNN50 12 hours after birth [Срок оценки: 12 hours after cesarean birth]
• Neonatal RMSSD 24 hours after birth [Срок оценки: 24 hours after cesarean birth]
• Neonatal SDNN 24 hours after birth [Срок оценки: 24 hours after cesarean birth]
• Neonatal pNN50 24 hours after birth [Срок оценки: 24 hours after cesarean birth]