Effect of Melatonin in Patients With Diabetic Peripheral Neuropathy

Diabetic neuropathy is a microvascular complication affecting 50% of patients throughout their lifetime. It leads to various complications including foot ulcerations and lower limb amputations, thus impairs the patient's quality of life. Distal symmetrical polyneuropathy is the most common subtype. Symptoms include tingling, burning and electrical pain associated with nocturnal exacerbation. Almost 50% of patients are asymptomatic thus diagnosis must include assessment of signs.

Pathophysiologic mechanism behind hyperglycemia induced nerve damage include the activation of various pathways including polyol and advanced glycation end product (AGE), which produces reactive oxygen species. Molecular studies have revealed the involvement of certain transcriptional regulators such as Nrf2-Keap1 and NfKb inflammatory cascade.

Nuclear erythroid growth factor-2 (Nrf2) functions primarily as a defense mechanism in cellular oxidative stress, producing anti-oxidant enzymes as glutathione reductase (GSH) and superoxide dismutase (SOD). However, in long term hyperglycemia, downregulation of nrf2 takes place, leading to accumulation of reactive oxygen species (ROS), which in turn activates nfkb inflammatory pathway and produces various cytokines such as tumor necrosis factor-alpha (Tnf-alpha), interleukin-1 (IL-1) and interleukin-6 (IL-6). Tumor-necrosis factor is known to be highly associated with nerve damage.

Drugs that target disease pathophysiology are currently unavailable, instead DPN management mainly depends on lifestyle modification through weight loss, blood pressure, blood glucose, lipid profile management along with symptomatic care. Yet these fail to stop disease progression.

Melatonin is known to possess anti-oxidant and anti-inflammatory properties through upregulating nrf2. It's hypothesized that adding it to standard therapy shall activate nrf2, and downregulate nfkb pathway and inflammatory cytokines, improving disease progression and patient's quality of life.

The aim of the current study is to measure the effect of melatonin on oxidative stress, inflammatory markers and clinical outcome in type 2 diabetic patients with DPN.

All patients will be assessed for the following data at baseline:

1. Age, gender, diabetes duration, diabetic neuropathy duration. 2. HbA1c, liver functions, kidney functions and lipid profile.

Lab assessment will be done at both baseline and at the end of the trial to assess the effect of melatonin on the following:

1. Nuclear erythroid related factor-2 (NRf2) (an oxidative marker) 2. Tumor necrosis factor-alpha (an inflammatory marker)

Assessment of clinical outcomes will be done as well, at both baseline and endpoint using the following:

1. Toronto clinical scoring system (TCSS) 2. Michigan neuropathy screening instrument questionnaire (MNSIQ) Samples used to assess oxidative and inflammatory markers will be stored at -80 degrees to be analyzed later at the end of the study using ELISA kits.

• Concentration of Tumor necrosis factor alpha [Срок оценки: Change from baseline Tumor necrosis factor alpha at 3 months]
• Michigan neuropathy screening instrument questionnaire (MNSI-Q) [Срок оценки: At baseline and after 3 months]
• Toronto clinical scoring system (TCSS) [Срок оценки: At baseline and after 3 months]
• Diabetic neuropathy score (DNS) [Срок оценки: At baseline and after 3 months]
• D-39 questionnaire [Срок оценки: At baseline and after 3 months.]