Autologous Serum Obtained by a Closed-Circuit Collection Device

Autologous Serum exhibits characteristics very similar to those of tears, such as pH, osmolarity, vitamins, and Immunoglobulin A. It also contains growth factors, nutritional factors, and antibacterial components that are necessary for the maintenance of cellular viability in the epithelial repair process. The use of autologous serum eye drops was first described in 1984 by Fox et al., in research for a preservative-free tear substitute. Subsequently, in 1999, Tsubota et al. found that, due to the presence of growth factors and vitamins, autologous serum could have a true epithelial trophic potential for the ocular surface. The autologous serum eye drops are not only a lubricant for the ocular surface but also provide various essential substances for the reconstruction of epithelial damage, including vitamin A, epithelial growth factor, fibronectin, and a variety of cytokines. With these epithelial trophic factors, autologous serum facilitates proliferation, migration, and differentiation of the ocular surface epithelium. Moreover, it is known for its anti-catabolic properties, inhibiting the inflammatory cascade triggered by interleukin-1, which prevents tissue destruction. Therefore, autologous serum eye drops have been effective in the treatment of persistent epithelial defects, neurotrophic ulcers, superior limbic keratoconjunctivitis, dry eye conditions, graft-versus-host disease (GVHD), or after refractive surgeries, such as LASIK (Laser Assisted In Situ Keratomileusis). In 2020, Wang et al. published an article with 7 randomized controlled trials comparing the use of autologous serum versus artificial tears in patients with dry eye syndrome. In the meta-analysis, all 7 studies evaluated subjective symptoms and showed that autologous serum eye drops were superior to ocular lubricants in alleviating and remitting symptoms. It was shown that autologous serum eye drops significantly improved parameters such as OSDI (Ocular Surface Disease Index), tear break-up time, and Bengal Rose staining when compared to the control group using ocular lubricants. Given the numerous properties of autologous serum eye drops, there is no doubt about their benefit and effectiveness in treating several ocular surface diseases, including ocular GVHD. However, the difficulty in accessing production and the substantial cost of autologous serum eye drops are the main challenges, and their use is often limited to more severe dry eye cases and those refractory to conventional treatment.

• Median pre-freezing number of leukocytes in autologous serum [Срок оценки: 2 years]
• Median pre-freezing number of red blood cells in autologous serum [Срок оценки: 2 years]
• Median pre-freezing number of platelets in autologous serum [Срок оценки: 2 years]
• Median pre and post-freezing pH in autologous serum [Срок оценки: 2 years]
• Pre-freezing microbiological test of autologous serum [Срок оценки: 2 years]
• NIH ocular score [Срок оценки: baseline, 6 weeks, 12 weeks, and end of treatment]
• Ocular Surface Disease Index [Срок оценки: Baseline, 6 weeks, 12 weeks, and end of treatment]
• Global ocular symptom score [Срок оценки: Baseline, 6 weeks, 12 weeks, and end of treatment]
• Lee score ocular subscale [Срок оценки: Baseline, 6 weeks, 12 weeks, and end of treatment]
• Corneal staining score (fluorescein) [Срок оценки: Baseline, 6 weeks, 12 weeks]
• Corneal staining score (Rose Bengal) [Срок оценки: Baseline, 6 weeks, 12 weeks]
• Meibography [Срок оценки: Baseline, 6 weeks, 12 weeks]