Effects of Ketones on Brain Energetics and Alcohol Consumption in Alcohol Use Disorder

The purpose of this research is to study how a nutritional ketone supplement, Kenetik may affect brain function and alcohol consumption in individuals with alcohol use disorder.

The study will use two types of imaging to see how the brain responds to Kenetik versus a placebo beverage. The study will use magnetic resonance imaging (MRI) and fludeoxyglucose-18 (FDG) positron emission tomography (PET) scans to study the brain. MRI scans use large magnets to create a magnetic field and radio waves to produce images of the brain. The FDG PET/CT scans use small amounts of a radioactive imaging drug, a special camera, and a computer to evaluate brain and tissue functions. FDG PET/CT scans are routinely done to evaluate brain and other organ diseases. The study will test brain functions at rest and while viewing pictures of alcohol. Participants will be asked to complete 2 MRI scans and 2 PET scans. Participants will randomly receive the Kenetik at 1 MRI scan and at 1 PET scan and will receive the placebo at the other MRI and PET scan visits.

The study will use two alcohol bar labs to study how Kenetik may affect alcohol consumption. Participants will randomly receive Kenetik on one of the visits, and the placebo beverage at the other visit. At each bar lab, Participants will be asked to drink alcohol beverages.

The study is double-blinded, which means that participants and the study staff running the study visits will not know at which visit participants will receive the Kenetik or placebo beverage.

Visit 1. Consent and Screening Visit (this will last \~4-5 hours)

Participants are asked not to eat or drink anything except water on the day of the screening session. A a meal will be proved during the visit. A breathalyzer test will be given to ensure that participants breath alcohol levels are 0.020 or lower and are sober and able to make a well-reasoned decision about whether or not to participate in the study.

The following will occur at the screening session, following signed consent:

* Demographic information, such as date of birth, age, biological sex, race, and ethnicity will be collected. * Current information about address and contact information to into the University of Pennsylvania electronic medical record system. * Provide a urine sample of about 30 mL (approximately 2 tablespoons) to be used for a drug screening test. Results from this testing are used for research purposes only. Participants will be informed of eligibility status after testing, but specific results will not be shared. * Woman of childbearing potential, will be tested for pregnancy. * Study personnel will measure vital signs (blood pressure and pulse), weight, and height. A doctor or nurse practitioner will do a brief physical exam. * Staff will ask about any vision impairment or concerns about the ability to complete tasks or questionnaires on a computer. * Staff will interview participants with a brief psychiatric structured interview called the 'MINI'. The interviews will ask about any current and past drug and alcohol use, depressed mood symptoms, as well as other psychiatric symptoms, including suicidal thinking. * Participants will complete a brief mental ability test called the 'Shipley Institute of Living Scale'. * Participants will complete a paper and pencil assessment or a short interview regarding alcohol and smoking history, behaviors, mood, and any drug or alcohol use. Additionally participants will be asked about any substance cravings or withdrawal symptoms. * Participants will complete several psychological questionnaires related to substance use, cravings, risk behaviors, mental health, personality, depression and anxiety, and quality of sleep. * Participants will complete questions about any medications, recreational drugs, or supplements they may be taking. * Participants will complete a questionnaire about the presence of metal in their body to determine if it is safe to undergo an MRI. * A small amount of blood (approximately 2.75 tablespoons) will be drawn for screening lab tests (a blood count and chemistry panel). * Staff will perform an oral swab rapid HIV test. Staff will inform participants of the results and provide information about HIV. If the test is positive, study staff will draw a tablespoon of blood to conduct a confirmatory HIV blood test (i.e., to confirm the positive oral test). A clinician will discuss the results of this test. If the blood test is positive for HIV, staff will provide a referral for medical care and a copy of the test to provide to the doctor. Participants who tests positive for HIV will not be eligible to enroll in this study, because even without symptoms of HIV, the infection can make it impossible to read the MRI test results accurately. * Participants will complete several computer-based tasks on a laptop that will be provided by the study team. These tasks will test the following brain cognition measures including visual memory, willingness to delay a reward offered, response time in reacting to specific information on the screen, verbal learning and memory, impulsiveness, and reaction time and accuracy when asked to match up a series of test pictures that are different shapes and colors. The computer tasks will take you about 50 minutes to complete.

After the screening visit, staff will call participants to confirm that the study is a good fit and schedule the rest of the study visits.

Visits 2 \& 3. Alcohol Bar Lab visits (approximately 5 hrs.) The following will occur on the day of the two bar lab visits for participants. Participants will not to eat or drink anything except water after midnight the day before and on the day of the lab visit.

* A Breathalyzer test to measure breath alcohol concentration. Participants are required to have a 0.000% BrAC to continue with the visit. Staff will perform a finger stick to test glucose and BHB levels, using an instant-read point of care meter. Staff will provide the glucose level and what the reading means. Staff will take weight and vital signs. * Staff will ask about recent use of drugs, alcohol, nicotine, and substance use and then ask for a urine sample. This will be used for a rapid urine drug test, and if the test is positive continued participation in the study will be reviewed by a study doctor. For woman of childbearing potential, a small amount of the urine will be used for a pregnancy test. The result of the pregnancy test must show not pregnant for to continue to participate in the study. * Staff will provide a light meal and participants will complete some questionnaires. * Staff will review the current list of medications and vitamins provided at the screening visit and ask if anything has changed * Approximately 30 minutes after eating, participants will be asked to drink the Kenetik beverage or placebo beverage within 15 minutes. Neither participants nor our staff will know which beverage was received. * Approximately 30 minutes after drinking the Kenetik or placebo beverage, participants will drink an alcoholic beverage based on weight, within 15 minutes. This drink will bring BrAC to approximately 0.050%. Staff will record the BrAC, and ask participants to complete the questionnaire as listed in the below table. * Staff will ask about any side effects that may occur throughout the lab procedures. * Alcohol bar lab (\~2 hours) Approximately 1 hour after the first dose of alcohol, staff will provide chips that equal $24 and that can be used to purchase more alcoholic beverages (8 mini drinks in total). Participants can decide to keep the money or use it to buy more drinks. Participants are required to stay in the lab room for \~2.5 hours in total. They can watch videos and read magazines during these hours. * After the meal, staff will check BrAC, BHB, and Glucose a final time. Ask about any side effects and staff will schedule the second lab visit. The second visit will occur at least 2 days after this visit and will follow the same procedures. Participants may leave once their BrAC is below 0.010. * Table of Lab Visits Approximate study times Procedure 1:00 PM Arrive at CSA 1:00-1:30 PM BrAC1, weight, vital signs, urine sample, finger stick for BHB, and Glucose, review of medications currently taking, and review of alcohol and drug use since last visit 1:30-2:00 PM light meal, and questionnaires1 2:00-2:15 PM Drink Kenetik or placebo beverage 2:15-2:30 PM BRAC2, and questionnaires2 2:30-2:45 PM Alcohol administration 2:45 PM BrAC 3 3:00 PM BrAC 4 3:15 PM BrAC 5, and Finger stick for BHB, and Glucose Ask about 3:30 PM BrAC 6, 3 questionnaires, Bar lab: first tray of 4 alcoholic mini-drinks.

4:30 PM BrAC 7, 3 questionnaires, Bar lab: second tray of 4 alcoholic mini-drinks.

5:00 PM BrAC 8 5:30 PM BrAC 9, and Study Intervention questionnaire 5:50 PM Meal, finger stick for BHB, and Glucose 6:00 PM BrAC 10, meet with study clinician and leaves by Lyft when BRAC is \<0.010% and Glucose returns to normal levels of \> 70 mg/dl.

\*Time in the above table may vary due to delays in start time of the lab visit.

Follow-up Visit (approximately 5 minutes): A follow-up phone call will occur the day after each lab visit and the following will occur:

• Staff will ask about any side effects that may have been experienced.

Visit 4 \& 5. MRI Scan visits (approximately 2 hours)

On the day before the scan visits, participants will not drink any alcoholic beverages after 10 PM. Staff will provide participants with a snack after the scanning visit is complete.

On the day of the study visits the following will be done:

* Meet a study team member at the Perelman Center for Advanced Medicine and complete the following tests: * A Breathalyzer test to measure breath alcohol concentration. A 0.000% BrAC is required to continue with the visit. Study personnel will measure vital signs (blood pressure and pulse), weight, and height. Participants will provide a urine sample for urine drug testing and urine pregnancy for woman of childbearing potential. A positive drug test result will be reviewed by the PI for continued participation in the study. * Staff will ask about any changes in any of the medications or vitamins that was reported since the last visit. Staff will be asked about recent use of alcohol, drugs, and nicotine since the last visit. * Participants will drink the Kenetik beverage or placebo beverage within 15 minutes. Neither participants nor our staff will know which beverage was received. * Participants will receive a brain 7-Tesla MRI scan (\~1.5 hours). An MRI is a type of scan that uses radio waves to take detailed pictures of the body. Participants lie on an MRI table where the technologist will place a receiver on the part of the body to be studied (brain). A blanket will be provided for comfort and earplugs since the MRI makes noises while it is scanning. Participants will still be able to hear some sound to ensure communication with the technologist and follow any direction given throughout the MRI scan. The technologist will slowly slide participants into the MRI magnet where radio waves will be transmitted.

* Participants will fill out an MRI safety form. * Participants will remove any metal they may be wearing. * Staff will provide earplugs for the noise from the MRI scanner, and positioning on the imaging table with head in the scanner, held comfortably in a special head holder. * Staff will provide instructions not to move the head and to lie as still as possible during the scan (\~ 90 minutes). There will be several scans, during which staff will obtain a structural image of the brain, and measure levels of ketones (energy in the brain), neurotransmitter GABA and glutamate, and NAD (a cofactor involved in energy metabolism). * After the MRI visit is complete staff will provide a light snack and participants may leave. Staff will call the next day to inquire about any side effects of the MRI day scan.

Visit 6 \& 7. PET Scanner Visits (this will last 3 h

• To determine the effects of KS compared to placebo on alcohol pharmacokinetics and subjective responses following an alcohol priming dose. [Срок оценки: 3 hours]
• To determine the effects of KS compared to placebo on alcohol consumption. [Срок оценки: 2 hours]