Antibacterial Effect of Zinc Oxide Nanoparticles on Acinetobacter Baumannii Isolated from Patients with Hospital Acquired Infections in Sohag University Hospitals, Egypt

Обзор

A. baumannii is known as the most frequently isolated organism in intensive care units (ICUs), causing a variety of nosocomial infections, including pneumonia, urinary tract infections (UTIs), bacteremia as well as skin and soft tissue infections. These infections are usually associated with high mortality rates ranging between 26% among hospitalized patients and 43% among ICU patients.

Подробное описание

Acinetobacter baumannii (A. baumannii) is Gram- negative, aerobic, glucose non-fermentative, non-motile coccobacillus, ubiquitous in nature, and persistent in healthcare settings. It is regarded as a significant opportunistic human pathogen.

This bacterium become a growing problem in hospitals as a predominant multidrug-resistant (MDR) bacterium. Horizontal acquisition of resistance genes is the main factor involved in the emergence of MDR .

There are many mechanisms to confer resistance to different classes of antibiotics in A. baumannii, one of them is multidrug efflux pumps. These efflux pumps are important source of MDR, which export antibiotics from the cell, increasing their antibiotic resistance.

AdeABC is one of the most important efflux systems, belonging to the RND family in Acinetobacter, which plays an important role in the resistance to a broad group of antibiotics; its genes are chromosomal and encode three genes, i.e., AdeB, AdeA, and AdeC, forming an operon in the vicinity. In addition, the expression of AdeABC is done by a two-component system, which includes a response regulator (AdeR) and a sensor kinase (AdeS) .

The ability of Acinetobacter spp. to form biofilm that enables bacterial survival in hospital settings, especially in ICUs, is the most significant contributing factor to their virulence, and this trait is also responsible for their notable antibiotic resistance. Several biofilm-related genes influence antimicrobial susceptibility, suggesting an association between the biofilm-forming ability of Acinetobacter spp. and their antibiotic resistance patterns (MDR/XDR)

Zinc oxide nanoparticles (ZnO NPs) are one of the most important nanoparticles of metal oxides; it is a unique and inorganic materials that can be used in several biological applications (anti-bacterial, anti-inflammatory). ZnO NPs exhibit distinctive properties other than other nanoparticles such as higher solubility, better biofilm penetration and effective drug delivery . ZnO NPs have been reported to have antimicrobial properties such as disrupting the cell membrane of pathogens, accumulating in the cell and producing toxic H2O2 (hydrogen peroxide)

Вмешательства

• Диагностический тест Culture specific antibiotic therapy
  Samples will be cultured on MacConkey agar. Morphological identifications of growth isolates by Gram staining, colony features and conventional biochemical tests. All isolates will be identified to species level using automated bacterial identification systems.
• Диагностический тест Antibiotic
  Strains confirmed as A. baumannii will be examined for their different antibiotic susceptibility by modified Kirby Bauer\'s disc diffusion method on Mueller Hinton Agar.
• Диагностический тест Biofilm assessment
  The biofilm formation activity of A. baumannii isolates will be tested using the microtitre plate technique
• Диагностический тест nanoparticle zinc oxide
  Detection of the effect of ZnO NPs on the biofilm producer MDR A. baumannii strains using the same method
• Диагностический тест Molecular detection of antibiotic resistance
  \- Evaluation the effect of ZnO NPs on the expression of some efflux pump genes, and biofilm related genes in MDR A. baumannii isolates using Real time PCR technology.

Первичные конечные точки

• A. baumannii antibiotic susceptibility profile [Срок оценки: October 2024 to December 2025]
• Isolation and identification of A. baumannii from different clinical samples [Срок оценки: October 2024 to December 2025]
• A. baumannii strains which produce biofilm [Срок оценки: October 2024 to December 2025]
• Ability of ZnO NPs to inhibit the phenomenon of biofilm formation by MDR A. baumannii strains [Срок оценки: October 2024 to December 2025]
• The effect of ZnO NPs on the expression of some efflux pump genes and biofilm related genes in MDR A. baumannii strains [Срок оценки: October 2024 to December 2025]

Критерии участия

Критерии включения

• All patients suffering from infections that can be caused by A. baumannii.

Критерии исключения

• All patients suffering from infections that aren\'t caused by A. baumannii.

Критерии приведены из реестра в оригинале (на английском). Окончательную оценку соответствия проводит исследовательский центр.

Здоровые добровольцы: Нет

Дизайн исследования

Модель наблюдения

Случай-контроль

Центры проведения

Египет · 1 центр

• Sohag university, Faculty of medicine — Sohag

Идентификаторы

NCT: NCT06628076 · Soh-Med-24-9-1MD

Первоисточники (государственные реестры)

• ClinicalTrials.gov (NIH, США)

Открыть это исследование на ClinicalTrials.gov ↗