Diagnostic Performance of a Combination of Leukocyte Cell Surface Markers in Predicting the Risk of Severe Bacterial Infection in Febrile Children Under Three Months of Age in the Emergency Department: a Pilot Study.

Официальное название

Diagnostic Performance of a Combination of Leukocyte Cell Surface Markers in Predicting the Risk of Severe Bacterial Infection in Febrile Children Under Three Months of Age in the Emergency Department: a Pilot Study. ( CYTOFEB )

Обзор

Fever is a frequent reason for emergency department (ED) visits in infants under 3 months of age. Although viral infections are the most common etiologies, the prevalence of severe bacterial infections (SBI) is high (10%). While in infants with SBI, establishing the diagnosis and initiating rapid intravenous antibiotic therapy is necessary, every effort should be made to avoid it in those at low risk of SBI. Clinical examination and biomarkers are still sub-optimal for assessing the risk of SBI. As a result, the vast majority of these children receive inpatient intravenous antibiotic therapy. Flow cytometry is a technique for measuring the expression of biomarkers on the cell surface of leukocytes in response to infection. A French team has identified a combination of some fifteen leukocyte cell surface markers that perform excellently in discriminating between bacterial and viral infections in a population of adults presenting to the emergency department with a suspected infection. However, there are no similar studies in children. The objective of the study is to assess the diagnostic performance of a combination of biomarkers on the cell surface of leukocytes in discriminating between bacterial and viral infection. Infants less than 3 months of age, visiting the ED for fever will have an extra blood sample in order to measure those biomarkers. The performance of those biomarkers to identify SBI will be assessed.

Вмешательства

• Другое Blood sampling and follow-up call
  An additional 1.5 mL of blood will be collected at the time of the child care in the pediatric emergency department. In addition, a telephone call for patient follow-up (unless still hospitalized) will be done.

Первичные конечные точки

• Diagnostic performance of a combination of leukocyte cell surface markers for identifying IBS in febrile infants under 3 months of age presenting to emergency departments. [Срок оценки: At inclusion (D0)]

Критерии участия

Критерии включения

• Children aged between 7 and 90 days
• Visiting the emergency department with fever (Temperature greater than or equal to 38° measured rectally in the emergency department or reported by parents) OR whose fever is detected during the emergency department visit (Temperature greater than or equal to 38° measured rectally in the emergency department)
• Consent signed by one of the two parents/guardians

Критерии исключения

• Weight less than 2,500 grams
• Chronic illness (heart failure...)
• Known immune deficiency
• Congenital anomaly significantly modifying the probability of bacterial infection (pulmonary malformation, esophageal atresia...)
• Antibiotic therapy in the previous 48 hours
• Clinically evident source of fever: skin infection, joint infection, etc.
• Parents or guardians unable to understand French
• Non-affiliation with a social security scheme (including AME)

Критерии приведены из реестра в оригинале (на английском). Окончательную оценку соответствия проводит исследовательский центр.

Здоровые добровольцы: Нет

Дизайн исследования

Модель наблюдения

Когортное

Центры проведения

Франция · 2 центра

• Sorbonne University, pediatric emergency department, Trousseau hospital — Paris
• hospitalpediatric emergency department, Trousseau hospital — Paris

Идентификаторы

NCT: NCT06618989 · APHP231307

Первоисточники (государственные реестры)

• ClinicalTrials.gov (NIH, США)

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