Effects of Intravascular Administration of Mesenchymal Stromal Cells Derived from Wharton's Jelly of the Umbilical Cord on Systemic Immunomodulation and Neuroinflammation After Traumatic Brain Injury.

Обзор

Traumatic brain injuries (TBI) are one of the leading causes of death and disability worldwide. These patients are burdened by physical, cognitive, and psychosocial deficits, leading to an important economic impact for society. Treatments for TBI patients are limited and none has been shown to provide prolonged and long-term neuroprotective or neurorestorative effects. TBI related disability is linked to the severity of the initial injury but also to the following neuroinflammatory response which may persist long after the initial injury. Moreover, a growing body of evidence suggests a link between TBI-induced neuro-inflammation and neurodegenerative post traumatic disorders. Consequently, new therapies triggering immunomodulation and promoting neurological recovery are the subject of major research efforts. In this context, mesenchymal cell-based therapies are currently investigated to treat various neurological disorders due to their ability to modulate neuroinflammation and to promote simultaneous neurogenesis, angiogenesis, and neuroprotection. Clinical trials using intravenous MSC have been conducted for various pathologies, all these studies showing a good safety profile. The hypothesis of the study is that intravenous repeated treatment with MSC derived from Wharton's Jelly of the umbilical cord may be associated with a significant decrease of post-TBI neuroinflammation and improvement of neuroclinical status. The main objective of the study is to evaluate the effect of iterative IV injections of MSC on post-traumatic neuroinflammation measured in corpus callosum by PET-MRI at 6 months in severe brain injured patients unresponsive to simple verbal commands 5 days after sedation discontinuation.

Подробное описание

Traumatic brain injuries (TBI) are one of the leading causes of death and disability worldwide. These patients are burdened by physical, cognitive, and psychosocial deficits, leading to an important economic impact for society. Treatments for TBI patients are limited and none has been shown to provide prolonged and long-term neuroprotective or neurorestorative effects. TBI related disability is linked to the severity of the initial injury but also to the following neuroinflammatory response which may persist long after the initial injury.

Moreover, a growing body of evidence suggests a link between TBI-induced neuro-inflammation and neurodegenerative post traumatic disorders. Consequently, new therapies triggering immunomodulation and promoting neurological recovery are the subject of major research efforts.

In this context, mesenchymal cell-based therapies are currently investigated to treat various neurological disorders due to their ability to modulate neuroinflammation and to promote simultaneous neurogenesis, angiogenesis, and neuroprotection. Indeed, several experimental studies have reported that human umbilical cord-derived mesenchymal stromal cells (MSC) have the ability to improve neurological outcomes and recovery in cerebral injury animal models, including TBI.

Clinical trials using intravenous MSC have been conducted for various pathologies, all these studies showing a good safety profile. In TBI, small clinical trials using different modalities for administration of mesenchymal cells are available but none about MSC derived from Wharton's Jelly of the umbilical cord.

The hypothesis of the study is that intravenous repeated treatment with MSC derived from Wharton's Jelly of the umbilical cord may be associated with a significant decrease of post-TBI neuroinflammation and improvement of neuroclinical status.

The main objective of the study is to evaluate the effect of iterative IV injections of MSC on post-traumatic neuroinflammation measured in corpus callosum by PET-MRI at 6 months in severe brain injured patients unresponsive to simple verbal commands 5 days after sedation discontinuation.

Вмешательства

• Препарат Mesenchymal Stromal Cells (MSC)
  3 injections one week apart
• Препарат placebo
  3 injections one week apart

Первичные конечные точки

• effect of iterative IV injections of WJ-UC-MSC on post-traumatic neuroinflammation [Срок оценки: 6 months after the last injection]

Вторичные конечные точки (12)

• radiological markers from PET-MRI_1 [Срок оценки: 6 months after the last injection]
• radiological markers from PET-MRI_2 [Срок оценки: 6 months after the last injection]
• Treatment feasibility [Срок оценки: at the third injection]
• Neurological clinical Score M6 [Срок оценки: 6 months after the last injection]
• Neurological clinical Score M12 [Срок оценки: 12 months after the last injection]
• cognitive assessment M6 [Срок оценки: 6 months after the last injection]
• cognitive assessment M12 [Срок оценки: 12 months after the last injection]
• short term Tolerance D10 [Срок оценки: 10 days after the last injection]
• long term Tolerance M6 [Срок оценки: 6 months after the last injection]
• long term Tolerance M12 [Срок оценки: 6 months after the last injection]
• neuroinflammation of pericontusional [Срок оценки: 6 months after the last injection]
• neuroinflammation of grey matter [Срок оценки: 6 months after the last injection]

Критерии участия

20 healthy volunteers will be included for MRI normalization Volunteer eligibility criteria

Критерии включения

• Age 18-50 years
• ASA 1 classification (healthy patient)

Критерии исключения

• Lack of written consent
• Neurological history likely to alter the image (epilepsy, transient ischaemic attack, meningitis, head trauma)
• Vulnerable person according to article L1121-6 of the CSP
• Protected adult person
• No affiliation to a social security regime
• Pregnancy
• Contraindication for MRI and PET-MRI

• patients with Pacemaker and defibrillator
• MR-incompatible prosthetic heart valve
• Metallic intraocular, intra cerebral or intra medullary foreign bodies
• Implantable neurostimulation systems
• Cochlear implants/ear implant
• Metallic fragments such as bullets, shotgun pellets, and metal shrapnel
• Cerebral artery aneurysm clips
• Ventriculo peritoneal shunt with metallic component generating significant artefacts on the MR sequence
• Catheters with metallic components (Swan-Ganz catheter)
• Patient unable to remain supine and motionless during the duration of the examination

68 severe TBI patients with the following inclusion and exclusion criteria will be included"

Patient Inclusion criteria

• Age 18-50 years
• Severe TBI defined by:

• Glasgow score <12 within the 48 first hours,
• Brain traumatic lesion on CT scan,
• Need for intracranial pressure monitoring
• No other significant organ trauma (AIS <2)
• Unresponsive to verbal commands 5 days after sedation discontinuation, for whom, after usual clinical and paraclinical evaluation there has been no decision to interrupt active therapies within 10 days after sedation discontinuation
• Written consent signed by the close relative

Patient Exclusion criteria

• History of disease or treatment impairing current or previous year immunity function ( hematologic disease (leukemia, myeloma), viral disease affecting immunity (like HIV), immunological treatment (corticoid, anti rejection medication, anti TNFα, chemotherapy)
• History of severe neurological or psychiatric disease likely to alter neurological assessment
• HTAP > grade III OMS/WHO
• Ongoing uncontrolled infection with organ failure (septic shock, ARDS) including those due to severe COVID-19
• Platelets <100 G/L or <100000/μL, Hb <8 g/dL, lymphocytes count <1.5 G/L or 1500 μL , neutrophils count < 2.5G/L or <2500/μL, , creatinin > 100 μmol/L
• Liver function abnormalities (bilirubin> 2.5mg / dL or transaminases> 5x the ULN). Patients with Gilbert's disease are eligible if liver tests are normal excluding bilirubinemia
• Known HIV seropositivity
• Neoplasia ongoing or treated in the 3 years before screening
• Bone marrow transplant recipient
• History of transfusion reaction or hypersensitivity
• Pregnancy
• Contraindication for MRI and PET-MRI:

• Patient with Pacemaker and defibrillator
• MR-incompatible prosthetic heart valve o Metallic intraocular, intra cerebral or intra medullary foreign bodies
• Implantable neurostimulation systems o Cochlear implants/ ear implant
• Metallic fragments such as bullets, shotgun pellets, and metal shrapnel
• Cerebral artery aneurysm clips
• Ventriculo peritoneal shunt with metallic component generating significant artefacts on the MR sequence
• Catheters with metallic components (Swan-Ganz catheter)
• Patient unable to remain supine and motionless during the duration of the examination
• Participation in another interventional clinical trial of an investigational therapy within 30 days of consent
• No affiliation to a social security regime
• Vulnerable person according to article L1121-6 of the CSP
• Protected adult person

Критерии приведены из реестра в оригинале (на английском). Окончательную оценку соответствия проводит исследовательский центр.

Здоровые добровольцы: Да

Дизайн исследования

Распределение

Рандомизированное

Модель

Параллельные группы

Маскирование

Четверное слепое

Основная цель

Лечение

Центры проведения

Франция · 3 центра

• Hôpital National d'Instruction des Armées Percy — Clamart
• Beaujon Hospital — Clichy
• Hôpital de la Pitié Salpêtrière - AP-HP — Paris

Публикации

• Sigaut S, Tardivon C, Jacquens A, Bottlaender M, Gervais P, Habert MO, Monsel A, Roquilly A, Boutonnet M, Galanaud D, Cras A, Boucher-Pillet H, Florence AM, Cavalier I, Menasche P, Degos V, Couffignal C. Effects of intravascular administration of mesenchymal stromal cells derived from Wharton's Jelly of the umbilical cord on systemic immunomodulation and neuroinflammation after traumatic brain inj PMID 39740941

Идентификаторы

NCT: NCT06146062 · APHP211509 · 2021-006873-50

Первоисточники (государственные реестры)

• ClinicalTrials.gov (NIH, США)

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