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Not yet recruiting NCT07678346

Evaluation of AppleX™ Apple Extract on Anti-Inflammatory and Healthy Aging Effects

No phase Interventional Systemic Inflammation (hsCRP) Healthy Aging Vitality Healthy Adult Participants

For patients and families

In plain language

An automatic summary of structured registry data. It is an orientation aid, not a substitute for the official protocol or a physician assessment.

What is being studied
The protocol lists: Apple Extract, Placebo.
Who it may be relevant to
Registry conditions: Systemic Inflammation (hsCRP), Healthy Aging, Vitality, Healthy Adult Participants. Basic parameters: 45 years — 70 years · All.
What needs checking
Age, condition and sex are only basic indicators. Prior treatment, laboratory values and other mandatory requirements appear in the eligibility criteria below.
Where it takes place
United States
Next step
Save the trial, show it to the treating physician, and confirm current recruitment with the study center. Costs, documents and travel →
Official title

A Randomized, Double-Blind, Placebo-Controlled Pilot Study to Evaluate the Anti-Inflammatory and Healthy Aging Effects of AppleX™ Apple Extract (15% Fisetin) in Adults 45-70 Years of Age

Overview

The goal of this clinical trial is to learn if AppleX™ Apple Extract can help lower systemic inflammation in adults aged 45-70 with signs of low-grade inflammation. It will also look at the extract's effects on fatigue, joint comfort, and biological aging metrics. The main questions it aims to answer are: * Does taking AppleX™ Apple Extract daily lower high-sensitivity C-reactive protein (hsCRP) levels, a key marker of inflammation in the blood? * Does AppleX™ Apple Extract improve participant-reported fatigue and joint comfort? * Does the extract slow down biological or epigenetic aging metrics compared to a placebo? Researchers will compare AppleX™ Apple Extract to a placebo (a look-alike capsule that contains no active ingredients) to see if the apple extract has a measurable anti-inflammatory and healthy aging effect.

Detailed description

AppleX™ is a whole-apple extract standardized to 15% fisetin, delivering 15 mg of active fisetin within a natural polyphenol matrix of quercetin, chlorogenic acid, and procyanidins. This trial evaluates the physiological effects of continuous, lower-dose daily intake (100 mg/day) over a 24-week period, specifically targeting an aging population sample with mild systemic inflammatory burden.

The study utilizes a fully decentralized clinical trial framework. Biological metrics are collected remotely utilizing home-based, finger-prick dried blood spot (DBS) micro-sampling methodologies to minimize participant burden.

In addition to evaluating systemic inflammation through longitudinal tracking of high-sensitivity C-Reactive Protein (hsCRP), the protocol investigates cellular aging by assessing DNA methylation data. This exploratory analysis utilizes the TruAge testing platform (TruDiagnostic) to evaluate advanced biological clocks, including the DunedinPACE pace-of-aging algorithm and targeted organ-system sub-clocks focused specifically on immune and inflammatory aging profiles.

Interventions

  • Dietary supplement Apple Extract
    A whole-apple extract standardized to 15% fisetin within a natural polyphenol matrix of quercetin, chlorogenic acid, and procyanidins. Administered as a 100 mg capsule taken orally once daily with breakfast for 24 weeks.
  • Other Placebo
    An identical, matching inactive look-alike capsule containing microcrystalline cellulose. Administered orally once daily with breakfast for 24 weeks.

Primary outcome measures

  • Change from Baseline in High-Sensitivity C-Reactive Protein (hsCRP) at Week 24 [Time frame: Baseline (Week 0) and Week 24]
Secondary outcome measures (3)
  • Change from Baseline in Self-Reported Vitality and Energy Levels at Week 24 [Time frame: Baseline, Week 12, Week 24]
  • Change from Baseline in Self-Reported Fatigue at Week 24 [Time frame: Baseline, Week 12, Week 24]
  • Change from Baseline in Self-Reported Joint Comfort at Week 24 [Time frame: Baseline, Week 12, Week 24]

Eligibility criteria

Inclusion criteria

  • Age 45-70 years at time of enrollment
  • Meets questionnaire-based inflammatory enrichment criteria (at least 2 of 5 risk factors, per Section 4.2)
  • Non-smoker (no tobacco or nicotine use within 6 months of enrollment)
  • Willing and able to maintain stable diet, exercise habits, and supplement use throughout the 24-week study period
  • No major medication changes planned during the study period
  • Willing to perform home-based DBS collections at T0, T12, and T24
  • Access to a smartphone or computer to complete eConsent and online questionnaires
  • Able to provide informed consent via the Alethios eConsent platform

Exclusion criteria

  • Current use of prescription anti-inflammatory medications including NSAIDs (regular use ≥3x/week), corticosteroids, or DMARDs
  • Diagnosis of an autoimmune or inflammatory disease (e.g., rheumatoid arthritis, lupus, IBD, multiple sclerosis)
  • Active or unstable cardiovascular disease requiring medication changes during the study period
  • History of cancer within the past 5 years (except non-melanoma skin cancer)
  • Regular use of supplemental fisetin, quercetin, or resveratrol within 30 days of enrollment
  • Pregnancy, breastfeeding, or planning to become pregnant during the study
  • Known allergy to apple or apple-derived products
  • Body mass index (BMI) >40 kg/m²

Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.

Healthy volunteers: Yes

Study design

Allocation
Randomized
Model
Parallel assignment
Masking
Triple blind
Primary purpose
Basic science

Study locations

United States · 1 center
  • Alethios, Inc. — San Francisco

Identifiers

NCT: NCT07678346 · NLAX-001

Primary sources (government registries)

View this study on ClinicalTrials.gov ↗