Effects of Chlorhexidine on Human Gingival Fibroblasts
For patients and families
In plain language
An automatic summary of structured registry data. It is an orientation aid, not a substitute for the official protocol or a physician assessment.
- What is being studied
- This is an observational study: the protocol does not assign a study treatment.
- Who it may be relevant to
- Registry conditions: HGF, Fibroblast, Chlorhexidine, Cytotoxicity. Basic parameters: from 18 years · All.
- What needs checking
- Age, condition and sex are only basic indicators. Prior treatment, laboratory values and other mandatory requirements appear in the eligibility criteria below.
- Where it takes place
- Center list to be confirmed — check the primary protocol.
- Next step
- Save the trial, show it to the treating physician, and confirm current recruitment with the study center. Costs, documents and travel →
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Official title
In Vitro Effects of a New Chlorhexidine-based Solution on Human Gingival Fibroblasts Survival and Function
Overview
This prospective in vitro study aims to evaluate the effects of two chlorhexidine-based mouthwash formulations on the survival and function of human gingival fibroblasts (hGFs). Gingival fibroblasts obtained from discarded gingival tissue collected during routine dental procedures will be cultured and exposed to different concentrations of a conventional 0.2% chlorhexidine mouthwash and a 0.2% chlorhexidine mouthwash supplemented with N-acetylcysteine (NAC) and hyaluronic acid (HA). Cell viability, apoptosis, migration, and gene expression will be assessed at multiple time points using Trypan Blue exclusion assay, fluorescence-activated cell sorting (FACS), scratch assay, and quantitative real-time PCR. The study is designed to investigate the concentration-dependent effects of chlorhexidine on fibroblast viability and wound-healing-related functions and to determine whether the addition of NAC and HA may improve the biological compatibility of chlorhexidine-based formulations.
Primary outcome measures
- Human Gingival Fibroblast Viability [Time frame: 24 hours, 3 days and 7 days from the start of cell culture exposure]
Eligibility criteria
Inclusion criteria
- Male or female adults aged 18 years or older.
- Undergoing routine dental surgical procedures (e.g., tooth extraction or gingivectomy).
- Willing and able to provide written informed consent for the use of discarded gingival tissue for research purposes.
- Systemically healthy.
Exclusion criteria
- Presence of systemic diseases.
- Presence of relevant medical comorbidities.
- Current smoker.
- Refusal to provide informed consent for the use of discarded biological material.
Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.
Healthy volunteers: Yes
Study design
- Observational model
- Other
Study locations
Center list to be confirmed — check the primary protocol.
Identifiers
NCT: NCT07669636 · Chx.01