Effect of Smoking and Time of Day on Eosinophils
For patients and families
In plain language
An automatic summary of structured registry data. It is an orientation aid, not a substitute for the official protocol or a physician assessment.
- What is being studied
- The protocol lists: Smoking for 4 hours.
- Who it may be relevant to
- Registry conditions: Healthy. Basic parameters: 18 years — 50 years · All.
- What needs checking
- Age, condition and sex are only basic indicators. Prior treatment, laboratory values and other mandatory requirements appear in the eligibility criteria below.
- Where it takes place
- Germany
- Next step
- Save the trial, show it to the treating physician, and confirm current recruitment with the study center. Costs, documents and travel →
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Official title
Effects of Acute Smoking and Time of Day on Blood Eosinophils in Humans
Overview
The study investigates the effect of acute smoking and time of day on blood eosinophils in chronic smokers. Chronic cigarette smokers without chronic respiratory diseases smoking at least 8 cigarettes per day and never-smoking controls are examined. Blood eosinophil counts and surface molecules are measured at 8 and 12 am in both groups. In smokers, two measurements are performed (at least 4 weeks apart): one measurement with no smoking between 8 and 12 am, and another measurement with smoking of 8 cigarettes between 8 and 12 am.
Detailed description
20 chronic cigarette smokers without chronic respiratory diseases smoking at least 8 cigarettes per day and 20 never-smoking controls are recruited. Blood eosinophil counts and surface molecules are measured at 8 and 12 am in both groups. In smokers, two measurements are performed (at least 4 weeks apart, cross-over design): one measurement with no smoking between 8 and 12 am, and another measurement with smoking of 8 cigarettes between 8 and 12 am. The effects of the time of day (comparing 8 and 12 am) and the effects of acute smoking (comparing the effects of no smoking or acute smoking at 12 am) on blood eosinophil counts and surface molecules (using flow cytometry) are analyzed. Additional parameters examined: lung function, exhaled nitric oxide, total and specific IgE in serum.
Interventions
- Other Smoking for 4 hours
Smoking of 8 cigarettes in 4 hours
Primary outcome measures
- Blood eosinophil counts [Time frame: 8 am (baseline) and 12 am (follow-up after intervention)]
Secondary outcome measures (1)
- Blood eosinophil surface molecules [Time frame: 8 am (baseline) and 12 am (follow-up after intervention)]
Eligibility criteria
Inclusion criteria
- Healthy current smokers
- Age between 18-50 years
- Smoking of at least 8 cigarettes/day
Exclusion criteria
- Chronic respiratory diseases
- Any treatment with corticosteroids or other immunosuppressants
- Any acute illness
Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.
Healthy volunteers: Yes
Study design
- Allocation
- Randomized
- Model
- Crossover
- Masking
- Open label
- Primary purpose
- Basic science
Study locations
Germany · 1 center
- University of Rostock — Rostock
Identifiers
NCT: NCT07625111 · Smoke-Time-Eosinophils