Effect of Ceftazidime-Avibactam on Carbapenemase-Producing Klebsiella Pneumoniae
For patients and families
In plain language
An automatic summary of structured registry data. It is an orientation aid, not a substitute for the official protocol or a physician assessment.
- What is being studied
- The protocol lists: . - Genotypic detection of carbapenemase genes by conventional PCR..
- Who it may be relevant to
- Registry conditions: ISOLATION OF KLEB. by Culture on macConkey Agar, Antibiotic Sensitivity Testing of Different Antibiotics According to Clsi 25, Effect of Ceftazidime-Avibactam on Carbapenemase-Producing Klebsiella Pneumoniae, Detection of Resistance Gene in Isolates Using Conventional Pcr. Basic parameters: No limits · All.
- What needs checking
- Age, condition and sex are only basic indicators. Prior treatment, laboratory values and other mandatory requirements appear in the eligibility criteria below.
- Where it takes place
- Egypt
- Next step
- Save the trial, show it to the treating physician, and confirm current recruitment with the study center. Costs, documents and travel →
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Official title
Effect of Ceftazidime-Avibactam Against Carbapenemase-Positive Klebsiella Pneumoniae Causing Health Care-Associated Infections
Overview
Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups. Bacterial identification by Gram stain, culture and biochemical rections * analysisThe resistance pattern of the isolates will be detected by disc diffusion method. * phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes (KPC, VIM, IMP, OXA-48, NDM) by conventional PCR.
Detailed description
Carbapenem-resistant Enterobacterales (CRE) poses an urgent global public health threat, with more than 1,100 documented deaths, as reported in a 2019 antibiotic resistance publication by the U.S. Centers for Disease Control and Prevention.
Carbapenem-resistant Enterobacterales, including carbapenem-resistant Klebsiella pneumoniae (CRKP), is associated with higher mortality compared with infections caused by carbapenem-susceptible Enterobacterales infections Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups.
Bacterial identification by Gram stain, culture and biochemical rections
* analysisThe resistance pattern of the isolates will be detected by disc diffusion method. * phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes (KPC, VIM, IMP, OXA-48, NDM) by conventional PCR.
Interventions
- Genetic . - Genotypic detection of carbapenemase genes by conventional PCR.
Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups. Bacterial identification by Gram stain, culture and biochemical rections - analysisThe resistance pattern of the isolates will be detected by disc diffusion method. - phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes
Primary outcome measures
- 1 ISOLATION OF KLEB. by culture on macConkey agar [Time frame: 6 months from January to may]
- Antibiotic sensitivity testing of different antibiotics according to clsi 25 [Time frame: 6 months from January to may]
- Effect of Ceftazidime-Avibactam on Carbapenemase-Producing Klebsiella Pneumoniae [Time frame: 6 months from January to may]
- Detection of resistance gene in isolates using conventional pcr [Time frame: 6 months from June to December]
- Detection of some virulence genes using conventional pcr [Time frame: 6 months from June to December]
Eligibility criteria
Inclusion Criteria: Infections caused by klebsiella pneumoniae like skin infections, chest infections, surgical site infections, and urinary tract Infections.-
Exclusion criteria
- Infections caused by any organisms other than klebsiella pneumoniae and carbapenem resistant klebsiella pneumoniae by mechanism other than carbapenemases production.
Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.
Healthy volunteers: Yes
Study design
- Observational model
- Case-control
Study locations
Egypt · 1 center
- Faculty of medicine Sohag University — Sohag
Identifiers
NCT: NCT07369518 · Soh-Med-25-12-2MS