Effects of NR Supplementation on Metabolic Flexibility in Zone 2 Training
For patients and families
In plain language
An automatic summary of structured registry data. It is an orientation aid, not a substitute for the official protocol or a physician assessment.
- What is being studied
- The protocol lists: Nicotinamide Riboside (NR), Nicotinamide Riboside (NR).
- Who it may be relevant to
- Registry conditions: Health Adult Subjects. Basic parameters: 18 years — 80 years · All.
- What needs checking
- Age, condition and sex are only basic indicators. Prior treatment, laboratory values and other mandatory requirements appear in the eligibility criteria below.
- Where it takes place
- China
- Next step
- Save the trial, show it to the treating physician, and confirm current recruitment with the study center. Costs, documents and travel →
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Official title
Temporal and Dose-response Effects of Nicotinamide Riboside Supplementation on Metabolic Flexibility During Zone 2 Training in Adults: a Double-blinded, Randomized, Controlled, Crossover Trial
Overview
Zone 2 training, a low-intensity endurance exercise performed at approximately 66-80% of VO₂max or near the first ventilatory threshold, has gained popularity for improving aerobic capacity, fat metabolism, and mitochondrial efficiency. It preserves glycogen stores and delays fatigue, making it an essential training method for both athletes and recreational exercisers. Nicotinamide adenine dinucleotide (NAD) is a vital metabolite that regulates cellular energy metabolism and redox homeostasis. It participates in oxidative reactions within the tricarboxylic acid cycle and electron transport chain, sustaining ATP synthesis and mitochondrial efficiency. Exercise influences multiple pathways of NAD metabolism, altering the NAD⁺/NADH ratio and thereby affecting energy production and fatigue resistance. Studies have shown that both aerobic exercise and NAD precursor supplementation (e.g., nicotinamide riboside \[NR\]) can elevate body NAD levels and enhance mitochondrial function, though findings remain inconsistent due to variations in dosage and participants' characteristics. This study aims to investigate how acute NR supplementation interacts with Zone 2 cycling training in adults. Specifically, it examines the temporal and dose-dependent effects of NR supplementation on metabolic flexibility and exercise performance. The results may clarify the potential synergistic benefits of combining NAD supplementation with low-intensity endurance training to improve aerobic adaptability.
Interventions
- Dietary supplement Nicotinamide Riboside (NR)
600 mg NR - Dietary supplement Nicotinamide Riboside (NR)
300 mg NR
Primary outcome measures
- Substrate utilization (CHO and fat oxidation rate) [Time frame: From enrollment to the end of treatment at 4 weeks]
Secondary outcome measures (9)
- Oxygen consumption [Time frame: From enrollment to the end of treatment at 4 weeks]
- Heart rate [Time frame: From enrollment to the end of treatment at 4 weeks]
- Blood glucose concentration [Time frame: From enrollment to the end of treatment at 4 weeks]
- Blood lactate concentration [Time frame: From enrollment to the end of treatment at 4 weeks]
- Peak power output [Time frame: From enrollment to the end of treatment at 4 weeks]
- Mean power output [Time frame: From enrollment to the end of treatment at 4 weeks]
- Rating of perceived exertion [Time frame: From enrollment to the end of treatment at 4 weeks]
- Muscle pain scale [Time frame: From enrollment to the end of treatment at 4 weeks]
- Muscle fatigue scale [Time frame: From enrollment to the end of treatment at 4 weeks]
Eligibility criteria
Inclusion criteria
- Over 18 years old
- BMI <30 kg/m2
Exclusion criteria
- Present of health conditions that contraindicate participation in exercise training, such as a history of cardiovascular disease, cancer, or musculoskeletal disorders
- Currently taking vitamin B supplements and/or NAD precursors
- Taking medication that may affect the test results
Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.
Healthy volunteers: Yes
Study design
- Allocation
- Randomized
- Model
- Crossover
- Masking
- Double blind
- Primary purpose
- Other
Study locations
China · 1 center
- G02, Kwok Sports Building, The Chinese University of Hong Kong — Hong Kong
Identifiers
NCT: NCT07344636 · SBRE-25-0349