CD70-Targeted CAR-T Therapy in CD70-Positive Advanced Solid Tumors

Official title

Clinical Study of CD70-Targeted Chimeric Antigen Receptor T Lymphocytes (CAR-T) in Advanced CD70-Positive Malignant Solid Tumors

Overview

This study is a single-arm, open-label, dose-escalating + dose-expansion clinical study, aiming to evaluate the safety and efficacy of CD70-targeted CAR-T cell preparations, and to preliminarily observe the study drug in CD70-positive advanced malignant tumors. The pharmacokinetic characteristics of CAR-T cell preparations for the treatment of patients with CD70-positive advanced malignancies were obtained and the recommended dose and infusion schedule.

Detailed description

According to the different infusion methods, patients will be assigned to three parallel subgroups: intravenous infusion, intrapleural infusion, and intraperitoneal infusion.

Within each subgroup, the study is conducted in two sequential parts:

1. .Part A (dose-escalation): escalation begins at the lowest dose level; 3-6 subjects are enrolled at each dose level; 2. .Part B (dose-expansion): additional subjects are treated at the recommended dose identified in Part A to further evaluate safety and preliminary efficacy.

Interventions

• Biological CD70-targeted CAR-T cells
  Administration method: intravenous infusion. Subjects will receive conditioning therapy by Fludarabine and Cyclophosphamide before cell infusion.
• Biological CD70-targeted CAR-T cells
  Administration method: intrapleural infusion. Subjects will receive conditioning therapy by Fludarabine and Cyclophosphamide before cell infusion.
• Biological CD70-targeted CAR-T cells
  Administration method: intraperitoneal infusion. Subjects will receive conditioning therapy by Fludarabine and Cyclophosphamide before cell infusion.

Primary outcome measures

• To evaluate the safety of CAR-T cell preparations in the treatment of CD70-positive advanced malignancies [Safety and Tolerability] [Time frame: 1 month]
• Obtained the recommended dose and infusion regimen of CAR-T cells for the treatment of patients with CD70-positive advanced malignancies [Safety and Tolerability] [Time frame: 1 month]

Secondary outcome measures (4)

• Assessing disease control rates of CAR-T cell preparations in CD70-positive advanced malignancies [Effectiveness] [Time frame: 1 and 3 months]
• To characterize the in-vivo cellular kinetics of CAR-T cells【pharmacokinetics】 [Time frame: From infusion through Month 3]
• To characterize the in-vivo cellular kinetics of CAR-T cells【pharmacokinetics】 [Time frame: From infusion through Month 3]
• To assess the inflammatory response following CAR-T cell infusion [Time frame: From infusion through Month 3]

Eligibility criteria

Inclusion criteria

• Age ≥18 years, regardless of gender;
• Histologically or cytologically confirmed advanced/metastatic solid tumors (tumors with positive CD70 expression, confirmed histopathological ly with IHC 3+ score);
• Failed or intolerant to standard second-line treatments (at least one of the following: tyrosine kinase inhibitors (TKIs), poly(ADP-ribose) polymerase inhibitors (PARPi), anti-angiogenic therapy; disease progression or inability to tolerate surgery, chemotherapy, radiotherapy, or targeted therapy);
• At least one measurable lesion per RECIST 1.1 criteria, with measurable lesions defined as:

• Extranodal lesions with a long axis ≥10mm on CT scan;
• Lymph node lesions with a short axis ≥15mm on CT scan;
• CT slice thickness ≤5mm.
• ECOG performance status of 0-2 ;
• Expected survival ≥12 weeks;
• No history of severe psychiatric disorders;
• Adequate organ function as defined by the following:

• Hematology: White blood cell count >2.0×10⁹/L, neutrophils >0.8×10⁹/L, lymphocytes >0.5×10⁹/L, platelets >50×10⁹/L, hemoglobin >90g/L;
• Cardiac: Echocardiogram showing left ventricular ejection fraction (LVEF) ≥50%, and ECG with no significant abnormalities;
• Renal: Serum creatinine ≤2.0×ULN;
• Hepatic: ALT and AST ≤3.0×ULN (may be relaxed to ≤5.0×ULN in cases with liver tumor infiltration); total bilirubin ≤2.0×ULN (may be relaxed to ≤3.0×ULN in cases with Gilbert's syndrome or liver tumor infiltration);
• Oxygen saturation ≥92% without supplemental oxygen;
• Ability to undergo single or venous blood collection, with no contraindications to cellular collection;
• Female subjects must agree to use reliable contraception (excluding fertility awareness methods) from the time of informed consent until 1 year after CAR-T cell infusion;
• Subject or authorized guardian agrees to participate in the trial and signs the informed consent form (ICF), indicating understanding of the trial's purpose and procedures and willingness to participate.

Exclusion criteria

• Prior treatment with anti-CD70 therapies;
• Active/symptomatic central nervous system (CNS) metastasis or meningeal metastasis: Subjects with treated brain metastases are eligible if treatment was completed ≥4 weeks prior to screening and there is no evidence of progression on imaging;
• Prior treatments within specified time frames:

• Participation in other interventional clinical trials within 3 months before cell infusion (for unapproved drugs, the last dose must be ≥3 months prior; for approved drugs, ≥5 half-lives prior to cell infusion);
• Received chemotherapy or targeted therapy within 2 weeks prior to blood collection or within 5 half-lives of the drug (whichever is shorter);
• Received >10mg/day prednisone (or equivalent) within 2 weeks prior to blood collection, unless for adrenal replacement or inhaled/local steroids (except for active autoimmune disease);
• Received live attenuated vaccines within 4 weeks prior to screening;
• Active infection requiring systemic treatment or uncontrolled infection within 1 week before screening;
• History of any other malignancy within the past 3 years, except for treated and stable non-melanoma skin cancer or malignancies treated with curative intent and no evidence of active disease for ≥3 years;
• Cardiovascular conditions:

• NYHA Class III or IV heart failure;
• Myocardial infarction or coronary artery bypass graft (CABG) within 6 months prior to screening;
• Clinically significant ventricular arrhythmias or unexplained syncope (excluding vasovagal or dehydration);
• Severe non-ischemic cardiomyopathy;
• Active or uncontrolled autoimmune diseases such as Crohn's disease, rheumatoid arthritis, systemic lupus erythematosus, systemic vasculitis, etc.;
• Positive for HBsAg or HBcAb with elevated HBV DNA in peripheral blood; positive for HCV antibodies with detectable HCV RNA levels; positive for HIV antibodies; positive syphilis test;
• Toxicity from prior anti-tumor treatments has not resolved to baseline or ≤grade 1, except for alopecia or peripheral neuropathy;
• History of venous thromboembolism (e.g., pulmonary embolism) requiring ongoing anticoagulation treatment, or meeting one of the following criteria:

• Severe bleeding (grade 3 or 4) lasting for ≥30 days;
• Post-thrombotic sequelae (e.g., persistent dyspnea and hypoxia) due to venous thromboembolism;
• Pregnant or breastfeeding women;
• Other conditions that, in the opinion of the investigator, make the subject unsuitable for participation in the trial.

Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.

Healthy volunteers: No

Study design

Allocation

Non-randomized

Model

Parallel assignment

Masking

Open label

Primary purpose

Treatment

Study locations

China · 1 center

• The 901 Hospital of Joint Logistics Support Force of People Liberation Army — Hefei

Identifiers

NCT: NCT07181720 · PBC095

Primary sources (government registries)

• ClinicalTrials.gov (US NIH)

View this study on ClinicalTrials.gov ↗