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Recruiting NCT06887036

Methylene Blue Treatment of Chronic Hepatitis B Virus Infection

Phase II Interventional Chronic HBV Infection

For patients and families

In plain language

An automatic summary of structured registry data. It is an orientation aid, not a substitute for the official protocol or a physician assessment.

What is being studied
The protocol lists: Methylene Blue.
Who it may be relevant to
Registry conditions: Chronic HBV Infection. Basic parameters: 18 years — 79 years · All.
What needs checking
Age, condition and sex are only basic indicators. Prior treatment, laboratory values and other mandatory requirements appear in the eligibility criteria below.
Where it takes place
Switzerland
Next step
Save the trial, show it to the treating physician, and confirm current recruitment with the study center. Costs, documents and travel →
Official title

Methylene Blue Treatment of Chronic Hepatitis B Virus Infection: a Pilot Study

Overview

HBV infection is a global public health problem. It is estimated that there are more than 250 million HBV carriers worldwide, of whom approximately 600,000 die each year from HBV-related liver disease. Diagnosis and treatment depend on the stage of infection, the degree of liver inflammation, and the progression of fibrosis. Patients with HBeAg-positive or -negative hepatitis are usually treated with nucleos(-t)ide analogues. This treatment inhibits HBV reverse transcriptase and reduces viral replication, but does not affect HBsAg production. Treatment must be continued for life in most cases and is associated with high cumulative costs and the risk of development of resistance. Recently, it has been shown that patients who show a decrease in their HBsAg levels are more likely to become HBsAg negative, seroconvert and be able to stop antiviral treatment, which is the ultimate goal of treatment, but unfortunately rarely achieved. Methylene blue (MB) has been shown to have an effect on HBsAg levels in vitro, making it a promising test for a new treatment for HBV. This is an explorative pilot study, open label, dose-escalation, single center clinical trial on the efficacy of MB against HBV infection. Primary objective is to assess the efficacy of incremental doses of MB against hepatitis B virus in patients with chronic hepatitis B assessed by the reduction of both the HBsAg and HBV DNA levels after 6 months of treatment and 6 months of follow-up. Participant will be treated for 6 months with MB 100 mg capsules, and then followed up for a further 6 months. The initial dose of MB might be increased if the patient is a non-responder.

Interventions

  • Drug Methylene Blue
    Subjects will be initially treated with an initial dose of 200mg/day for 8 weeks at which point the investigators will assess the effect of the treatment. Those patients that obtain a "complete response" will continue their treatment up to a total of 24 weeks with the same dose. Those who do not reach a "complete response" will be exposed to the next higher dose of 400mg/day for the following 8 weeks and the investigators will then again assess the effect of the treatment. Those patients that

Primary outcome measures

  • Reduction >1 log10 in the starting HBsAg level and > 2 log10 in the starting HBV DNA PCR level [Time frame: From enrollment to the end of treatment at 6 months]
Secondary outcome measures (2)
  • Adverse Events [Time frame: From enrollment to the end of follow up period (12 months in total)]
  • Treatment adherence [Time frame: From enrollment to the end of follow up period (12 months in total)]

Eligibility criteria

Inclusion criteria

  • Signed informed consent
  • Age ≥ 18 and < 80 years
  • Microbiologically confirmed chronic Hepatitis B Virus infection (according to the old terminology "inactive HBV carrier")
  • HBsAg levels within 20-15'000 IU/ml measured at the at least twice at 6-18 months apart
  • HBV DNA PCR levels within 1'000-20'000 IU/ml measured at least twice at 6-18 months apart
  • Negative pregnancy test in women of child-bearing age

Exclusion criteria

  • Documented refusal to participate in the study
  • Known G-6-Phophatase deficiency
  • Treatment with a serotoninergic drug
  • Ongoing treatment with a nucleos(-t)ide treatment
  • Clinically relevant concomitant liver disease
  • GPT > 2xULN
  • Fibroscan of > 8.0 KPa obtained ≤ 12 months before Visit 0/Screening
  • HBeAg positivity
  • Anti HDV antibody positivity
  • Breastfeeding women

Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.

Healthy volunteers: No

Study design

Allocation
N/A
Model
Single group
Masking
Open label
Primary purpose
Treatment

Study locations

Switzerland · 1 center
  • Epatocentro Ticino — Lugano

Identifiers

NCT: NCT06887036 · Methylene Blue in chronic HBV

Primary sources (government registries)

View this study on ClinicalTrials.gov ↗