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Enrolling by invitation NCT06709976

ReceptIVFity & Immunology in ART

Observational Infertility (IVF Patients) Infertility Assisted Reproductive Technology

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An automatic summary of structured registry data. It is an orientation aid, not a substitute for the official protocol or a physician assessment.

What is being studied
The protocol lists: Vaginal microbiome swab, Menstrual Blood Collection via Menstrual Cup.
Who it may be relevant to
Registry conditions: Infertility (IVF Patients), Infertility Assisted Reproductive Technology. Basic parameters: 18 years — 42 years · Female.
What needs checking
Age, condition and sex are only basic indicators. Prior treatment, laboratory values and other mandatory requirements appear in the eligibility criteria below.
Where it takes place
Netherlands
Next step
Save the trial, show it to the treating physician, and confirm current recruitment with the study center. Costs, documents and travel →
Official title

Increasing the Accuracy to Predict Pregnancy Success After Assisted Reproductive Therapy (ART) by Combining the ReceptIVFIty Test with Immunological Parameters

Overview

The goal of this clinical non-invasive observational pilot study is to improve the prediction of pregnancy success after In Vitro Fertilisation (IVF)/IVF -Intracytoplasmic Sperm Injection (ICSI) treatment by examining the vaginal microbiome and the immunological profile of menstrual blood in women (18-42) years old) undergoing their first IVF/IVF/ICSI treatment. The main questions it aims to answer are: What is the ReceptIVFity profile (low, medium, or high) of the vaginal microbiome in these women? How do the endometrial-derived lymphocytes respond to different immune stimuli and microbiota? Can the endometrial stromal cells decidualize effectively in the presence or absence of specific microbiota? Participants will: Self-perform a vaginal swab to determine the ReceptIVFity profile of their vaginal microbiome. Self-collect menstrual blood during 24 hours in 2 blocks of 12 hours for immunological analysis.

Interventions

  • Device Vaginal microbiome swab
    All swabs were collected using FLOQSwabs™ (Copan Italia SpA, Brescia, Italy). The patients were instructed to spread the labia with one hand, insert the swab 3-5 cm beyond the vaginal orifice with the other hand, and rotate the swab along the vaginal wall for 10-15 seconds.
  • Device Menstrual Blood Collection via Menstrual Cup
    Self-collect menstrual blood during 24 hours in 2 blocks of 12 hours

Primary outcome measures

  • Immune Cell Phenotype and Function in Menstrual Blood Stratified by Vaginal Microbiome in IVF Patients [Time frame: Baseline and 3 months]
  • Genotyping of Maternal KIR and HLA-C [Time frame: Baseline]
  • RNA Analysis of Stromal Fibroblast Cells [Time frame: Baseline]
Secondary outcome measures (1)
  • Metabolite Analysis as Immune Signaling Modulators in IVF Patients [Time frame: Baseline]

Eligibility criteria

Inclusion criteria

  • Indication for an IVF or IVF/ICSI procedure.
  • 18 years - 42 years.
  • European origin
  • Willing to provide a vaginal swab with the ReceptIVFity-test.
  • Willing to provide informed consent.

Exclusion criteria

  • No transferable embryos after IVF or IVF/ICSI.
  • Emergency IVF for cancer or other reasons.
  • Women with endometriosis pre-treated with an Gn-RH analogue.
  • The use of (hormonal) contraceptives or antibiotics 3 months prior to start IVF or IVF/ICSI.
  • Women unable or unwilling to agree with the procedures.
  • Women unable or unwilling to give written informed consent.

Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.

Healthy volunteers: Yes

Study design

Observational model
Cohort

Study locations

Netherlands · 1 center
  • Erasmus Medical Center — Rotterdam

Identifiers

NCT: NCT06709976 · OZBS72.19236

Primary sources (government registries)

View this study on ClinicalTrials.gov ↗