Effect of Different Centrifuge Values on Saliva High Sensitive C-Reactive Protein (Hs-CRP) Levels in Individuals With Different Smoking Amounts
For patients and families
In plain language
An automatic summary of structured registry data. It is an orientation aid, not a substitute for the official protocol or a physician assessment.
- What is being studied
- The protocol lists: saliva obtaining.
- Who it may be relevant to
- Registry conditions: Smoking. Basic parameters: 18 years — 30 years · All.
- What needs checking
- Age, condition and sex are only basic indicators. Prior treatment, laboratory values and other mandatory requirements appear in the eligibility criteria below.
- Where it takes place
- Turkey (Türkiye)
- Next step
- Save the trial, show it to the treating physician, and confirm current recruitment with the study center. Costs, documents and travel →
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Official title
Evaluating the Effect of Smoking Amount on Determining Salivary High Sensitive C-Reactive Protein(Hs-CRP) Values by Centrifuging the Saliva of the Same Individuals at Different Rpm and Time.
Overview
The purpose of this study; In periodontally healthy individuals, high sensitive c-reactive protein (hs-CRP) levels of non-smokers, smokers of less than 10 cigarettes per day and smokers of more than 10 cigarettes per day were measured at 15 rpm-10 min, 10 centrifuge values, which are frequently used in saliva studies. To determine the levels in saliva samples as a result of rpm-10 min and 10 rpm 6 min centrifugations and to determine the values at which a cytokine of this molecular weight should be centrifuged and to examine the possible correlation between these values and the clinical parameters of the amount of smoking. Materials and Methods: saliva samples were collected from 90 systemically healthy individuals who were non-smokers (N, n=30), light smokers \<10 cigarettes per day (L, n=30) and heavy smokers \>10 cigarettes per day (H, n=20). samples were taken. Full-mouth clinical periodontal measurements, including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI), and plaque index (PI), were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine hs-CRP levels in biological samples.
Interventions
- Diagnostic test saliva obtaining
The patient was asked to sit upright and tilt his/her head forward to collect saliva samples. İn this way, unstimulated saliva was allowed to accumulate in the floor of the mouth. The accumulated saliva was collected in a sterile container. It was then transferred to a propylene tube. The tubes were centrifuged and the clear part at the top of the tube was taken with a sterile syringe and transferred to a different propylene tube with 0.5 ml in each tube. Tubes were stored at -80ºC until the day
Primary outcome measures
- The total amount of hs-CRP in saliva [Time frame: 24 hours after taking the clinical measurements at the first visit]
Secondary outcome measures (1)
- Determining the ideal centrifugal value range of hs-CRP saliva level [Time frame: Within 1 week after receiving the biochemical analysis results]
Eligibility criteria
Inclusion criteria
- Systemically healthy
- Periodontally clinical healthy
- At least 20 permanent teeth in the mouth
- No medication for continuous use
- Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.
- Not in pregnancy or lactation period.
- Not received periodontal treatment in the last 6 months
- For the non-smoker group; person who does not smoke tobacco
- For the light-smoker group; person who smokes less than 10 cigarettes a day
- For heavy-smoker group; person who smokes more than 10 cigarettes a day
Exclusion criteria
- Any oral or systemic disease
- Regularly using a systemic medication
- During pregnancy or lactation
- Received periodontal treatment within the last 6 months.
- Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
- Individual with periodontal disease
Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.
Healthy volunteers: Yes
Study design
- Allocation
- Non-randomized
- Model
- Parallel assignment
- Masking
- Open label
- Primary purpose
- Diagnostic
Study locations
Turkey (Türkiye) · 1 center
- Izmir Katip Çelebi University Department of Periodontology — Izmir
Identifiers
NCT: NCT06561971 · 2024-SAEK-0029