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Recruiting NCT06543667

Limbal Stem Cell Derived Exosome (LSC-Exo) Eye Drop for Treatment of Dry Eye

Phase I Interventional Dry Eye Syndromes

For patients and families

In plain language

An automatic summary of structured registry data. It is an orientation aid, not a substitute for the official protocol or a physician assessment.

What is being studied
The protocol lists: Limbal Stem Cell Derived Exosomes Eye Drop.
Who it may be relevant to
Registry conditions: Dry Eye Syndromes. Basic parameters: 18 years — 70 years · All.
What needs checking
Age, condition and sex are only basic indicators. Prior treatment, laboratory values and other mandatory requirements appear in the eligibility criteria below.
Where it takes place
Iran
Next step
Save the trial, show it to the treating physician, and confirm current recruitment with the study center. Costs, documents and travel →
Official title

A Clinical Study for Safety and Efficacy Evaluation of Limbal Stem Cell Derived Exosome (LSC-Exo) Eye Drop for Treatment of Dry Eye Syndrome

Overview

The purpose of this study is to determine the safety and efficacy of Limbal Stem Cells derived Exosomes (LSC-Exo) in alleviation of dry eye symptoms

Detailed description

Dry eye is among the most prevalent chronic conditions in ophthalmology, significantly impacting quality of life and presenting a public health challenge that cannot be overlooked. Current treatment options primarily consist of artificial tear replacement, anti-inflammatory therapies, and local immunosuppressive treatments, which mainly focus on alleviating discomfort and other symptoms associated with the ocular surface. However, with the rapid advancements in regenerative medicine, ophthalmologists are exploring innovative approaches to treat dry eye. Chronic dry eye can result in corneal damage and potential vision loss. Recent studies suggest that therapeutic exosomes delivered as eye drops can enhance the immune microenvironment of the cornea and alter the ocular surface\'s microenvironment for better outcomes.

Exosomes are membrane vesicles secreted by parental cells, which can mediate the transfer of RNA, protein, DNA and other functional molecules of the parent cell between other cells and regulate the function of target cells. The therapeutic potential of Limbal Stem Cell Exosomes has been studied by our department. We found that LSC-Exo can alleviate the symptoms of dry eye in the animal study. At present, we plan to clarify its efficacy in a clinical research.

The main objective of this study is to evaluate the alleviation of Dry Eye symptoms in patients which are resist to conventional treatment by tear drops. The assessment will be done after LSC-Exo treatment by measuring Ocular Surface Index Score (OSDI), and the second objective include the measurement of inflammatory and pro-inflammatory cytokines in tear of patients, tear secretion amount, tear break time, the areas stained by fluorescent, ocular redness, tear meniscus and best corrected visual acuity.

Approximately 30 patients will be recruited. The treatment group will receive artificial tears for 2 weeks to normalize the baseline, followed by intervention of LSC-Exo 10ug/drop, four times a day for 30 days. The follow-up visit will be 12-weeks and the progression of dry eye will be measured.

Interventions

  • Biological Limbal Stem Cell Derived Exosomes Eye Drop
    Patients will receive artificial tears for 2 weeks to get the normalized baseline, followed by LSC-Exo eye drops 0.15 ml/single eye/one time, four times a day for 3 months. The follow-up visit will be 12 weeks since the administration of LSC-Exo eye drops.

Primary outcome measures

  • Change in Inflammatory and Pro-inflammatory cytokines changes [Time frame: 12 weeks]
  • Change in Ocular Surface Disease Index (OSDI) [Time frame: 4, 8 , and 12 weeks]
Secondary outcome measures (2)
  • Tear Secretion Test (Schirmer test) [Time frame: 4, 8 , and 12 weeks]
  • Tear Film Break-up Time [Time frame: 4, 8 , and 12 weeks]

Eligibility criteria

Inclusion criteria

  • Patients with complaints of burning or itching or feeling of a foreign body in the eye
  • Moderate and severe dry eye severity
  • Age between 18-70 years

Exclusion criteria

  • Previous history of eye surgery
  • Any disease that suppresses the immune system
  • Pregnancy and breastfeeding
  • Eyelid abnormalities
  • Use of eye drops in the past year except artificial tears and lubricating ointments
  • Use of systemic drugs that affect the tear film in last three months
  • Use of contact lenses in the last three months

Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.

Healthy volunteers: No

Study design

Allocation
N/A
Model
Single group
Masking
Open label
Primary purpose
Treatment

Study locations

Iran · 1 center
  • Moheb Kowsar Hospital — Tehran

Publications

  • Randall Harrell C, Djonov V, Volarevic A, Arsenijevic A, Volarevic V. Mesenchymal Stem Cell-Sourced Exosomes as Potentially Novel Remedies for Severe Dry Eye Disease. J Ophthalmol. 2025 Jan 10;2025:5552374. doi: 10.1155/joph/5552374. eCollection 2025. PMID 39839752

Identifiers

NCT: NCT06543667 · IRCT20231022059814N1 · 1358-06

Primary sources (government registries)

View this study on ClinicalTrials.gov ↗