The Role and Mechanism of GPER-Hippo-CBS/H2S Pathway in Preeclampsia
For patients and families
In plain language
An automatic summary of structured registry data. It is an orientation aid, not a substitute for the official protocol or a physician assessment.
- What is being studied
- The protocol lists: H2S content.
- Who it may be relevant to
- Registry conditions: Preeclampsia. Basic parameters: up to 40 years · Female.
- What needs checking
- Age, condition and sex are only basic indicators. Prior treatment, laboratory values and other mandatory requirements appear in the eligibility criteria below.
- Where it takes place
- China
- Next step
- Save the trial, show it to the treating physician, and confirm current recruitment with the study center. Costs, documents and travel →
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Official title
The Role and Mechanism of GPER Hippo Pathway in Regulating Uterine Arterial Smooth Muscle CBS/H2S in the Pathogenesis of Preeclampsia
Overview
The goal of this clinical trial is to learn about in health conditions. The main questions it aims to answer are: * The pathological significance of GPER in uterine artery dilation in preeclampsia * The Mechanism of GPER Hippo Pathway Regulating CBS/H2S in Human Uterine Artery Smooth Muscle Cells (hUASMC) This project intends to use GPER interfering RNA, YAP1 interfering RNA, in vivo perfusion experiments of human uterine artery tissue, and single cell patch clamp technology to study hypotheses under physiological/pathological pregnancy conditions at the tissue, cellular, and molecular levels, revealing a novel signal transduction pathway of estrogen stimulating vasodilation, providing new ideas for studying the mechanism of uterine artery blood flow regulation. This research result will provide new targets for intervention and treatment of diseases such as fetal intrauterine growth retardation and preeclampsia.
Detailed description
1. Measure the content of H2S in uterine arterial smooth muscle and circulation of normal pregnancy and PE pregnant women, and analyze the relationship between the content of H2S in uterine arterial smooth muscle and circulation of PE pregnant women and PE. 2. Knock down the GPER and overexpression of GPER in HTR-8 cells, and detect the expression of GPER, CBS, and Hippo pathway related proteins using Western blot and qRT-PCR to verify the efficiency of knockdown and overexpression. Use different concentrations of H2S donor (GYY4137) to interfere with HTR-8 cells after treatment, and use RT-PCR and Western blot to detect the mRNA and protein expression of downstream molecules YAP and TAZ in Hippo pathway in cells, respectively.
Interventions
- Other H2S content
H2S content
Primary outcome measures
- H2S content in uterine arterial smooth muscle circulation [Time frame: 8:00 am]
Eligibility criteria
Inclusion criteria
- Full term pregnancy with clinical diagnosis of preeclampsia and normal pregnancy pregnant woman
- Pregnant women undergoing lower segment cesarean section for delivery
Exclusion criteria
- Combined with chronic hypertension, kidney disease, intrahepatic cholestasis, etc Other basic diseases;
- Multiple pregnancy
- Pregnant women undergoing vaginal delivery
- Normal pregnant women who are<37 weeks or>40 weeks pregnant
Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.
Healthy volunteers: Yes
Study design
- Allocation
- Randomized
- Model
- Parallel assignment
- Masking
- Open label
- Primary purpose
- Basic science
Study locations
China · 1 center
- The First Affiliated Hospital of Shandong First Medical University &Shandong Provincial Qi — Jinan
Identifiers
NCT: NCT06205771 · YXLL-KY-2023（031）