Study of the Safety and Efficacy of an Adeno-Associated Viral Vector Carrying the SMN Gene After a Single Intravenous Administration of Escalating Doses in Children With Spinal Muscular Atrophy (BLUEBELL)

Official title

An Open-Label, Non-Comparative Clinical Study of the Safety and Efficacy of an Adeno-Associated Viral Vector Carrying the SMN Gene (ANB-004 (JSC BIOCAD, Russia)) After a Single Intravenous Administration of Escalating Doses in Children With Spinal Muscular Atrophy

Overview

The goal of this multicenter, open-label, non-comparative, cohort study is to investigate the safety, immunogenicity, and efficacy of ANB-004 in children with spinal muscular atrophy. The study will have a standard 3+3 dose-escalation design.

Detailed description

The study will be conducted in 2 stages:

Stage 1: pilot efficacy and safety study of different doses to select a potentially therapeutic dose for further study.

Stage 2: study of the efficacy and safety of ANB-004 at the selected potentially therapeutic dose.

At the first stage, the study will have a "3+3" design and involve dose escalation in two cohorts, with the possibility of including a third cohort. Three subjects are to be included in each cohort, each of whom will receive a pre-specified cohort dose of ANB-004 as a single intravenous infusion.

Subjects will be monitored for dose-limiting toxicity (DLT) events for 3 weeks after the drug infusion. In this study, DLT will include any of the CTCAE 5.0 grade 3 or higher adverse events (AEs) at least possibly related to the administration of the investigational product, except for an increase in body temperature at least possibly related to the administration of the investigational product, which will be classified as DLT starting from CTCAE 5.0 grade 4.

At the first stage, in the absence of DLT events in three subjects in the same cohort, an ID will be assigned, and an infusion will be administered to the first subject in subsequent cohort. If DLT events occur in 1 of 3 subjects in the same cohort, this cohort will additionally include 3 subjects who will receive the same ANB-004 dose with which the DLT event was observed. If DLT events occur in 2 or more of 3 subjects within the same cohort, the assignment of ID/infusion in subsequent subjects/study is suspended.

AT the second stage, if the IDMC judges that the potentially therapeutic dose was previously used in one of the cohorts, an additional 6 subjects will be included in this cohort.

Interventions

• Genetic ANB-004, dose 1
  Adeno-associated viral vector carrying the SMN gene single infusion at dose 1. The duration of the infusion is about 60 minutes.
• Genetic ANB-004, dose 2
  Adeno-associated viral vector carrying the SMN gene single infusion at dose 2. The duration of the infusion is about 60 minutes.
• Genetic ANB-004, dose 3
  Adeno-associated viral vector carrying the SMN gene single infusion at dose 3. The duration of the infusion is about 60 minutes.

Primary outcome measures

• Proportion of subjects with adverse reactions [Time frame: 12 months]
• Proportion of subjects with serious adverse reactions [Time frame: 12 months]
• Proportion of subjects with CTCAE 5.0 or DAIDS grade 3 or higher adverse reactions [Time frame: 12 months]
• Time from date of birth to event [Time frame: 12 months]
• Motor development score [Time frame: 12 months]
• Change in the The Hammersmith Infant Neurological Examination (HINE) score [Time frame: 12 months]

Secondary outcome measures (2)

• Event occurrence [Time frame: 12 months]
• Documented evidence of efficacy [Time frame: 12 months]

Eligibility criteria

Inclusion criteria

• Informed consent form for participation in the study signed by the subject's legal representative;
• Subjects of either sex under the age of 240 days at the time of signing the Information Sheet for the Legal Representative of the Clinical Study Subject with Informed Consent Form;
• A diagnosis of 5q-SMA (homozygous deletion of exon 7 of the SMN1 gene or heterozygous deletion of exon 7 + confirmed point mutation of the SMN1 gene) and 2 or 3 copies of the SMN2 gene established based on molecular genetic testing;
• Subjects with 2 copies of the SMN2 gene can be included in the study both at the presymptomatic stage of the disease and in the presence of SMA symptoms. If symptoms are present, the age of onset of the disease should be up to 180 days from birth.
• Subjects with 3 copies of the SMN2 gene can be included in the study if they have symptoms of SMA type 1 and the disease began before the age of 180 days.
• The ability of the subject's legal representative, in the Investigator's opinion, to perceive information and follow the Protocol procedures

Exclusion criteria

• A diagnosis of HIV infection, hepatitis B, hepatitis C, congenital syphilis in the study subject, as well as a documented diagnosis of HIV infection in the study subject's mother. Note: documented hepatitis B and/or hepatitis C and/or syphilis in the mother of a study subject is not an exclusion criterion in this clinical study, provided that standard breastfeeding rules are followed or the subject is not breastfed due to the low risk of transmission of hepatitis B and C viruses and Treponema pallidum from mother to child with breast milk;
• Unwillingness of the legal representative to use alternative feeding methods (nasogastric tube, gastrostomy) in case of swallowing disorders and a risk of aspiration;
• Anti-AAV9 antibody titer >1:50 determined by ELISA. Note: if a subject's screening anti-AAV9 antibody titer is >1:50, the anti-AAV9 antibody titer may be determined again. Subjects with anti-AAV9 antibody titers ≤1:50 in the second test may be included in the study;
• Need for respiratory support for ≥16 hours per day or tracheostomy ;
• Treatment with nusinersen, risdiplam, branaplam, onasemnogene abeparvovec or other antisense oligonucleotides/selective SMN2 splicing modifiers or gene therapy drugs for SMN1 transduction or other AAV-based gene therapy drugs regardless of serotype used previously (from birth) or planned for the main study period, i.e., within 12 months after the administration of the investigational product.
• A need to use any medications for the treatment of myopathy or neuropathy, drugs for the treatment of diabetes, ongoing immunosuppressive therapy, or the need for immunosuppressive therapy after the start of the study (for example, glucocorticoids (except for premedication and post-medication), cyclosporine, tacrolimus, methotrexate, cyclophosphamide, intravenous immunoglobulin, rituximab, etc.);
• Subjects with the following laboratory test results at screening:

• increased activity of transaminases (ALT, AST) or GGT >2×ULN;
• total bilirubin level ≥34 µmol/L;
• creatinine level ≥160 µmol/L;
• hemoglobin <80 g/L and >180 g/L;
• WBC count >20x109/L;
• Troponin I level > ULN.
• Any concomitant diseases that, in the Investigator's opinion, may affect the safety of ANB-004 in the subject or have a significant impact on the assessment of the outcomes of SMA therapy;
• A diagnosis of acute or chronic hepatic failure at screening;
• A known allergy or intolerance to any components of the investigational product or pre- and post-medication drug (glucocorticoids);
• Simultaneous participation of the subject in other clinical studies or previous participation in another clinical study using an experimental therapy.

Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.

Healthy volunteers: No

Study design

Allocation

Non-randomized

Model

Sequential

Masking

Open label

Primary purpose

Treatment

Study locations

Russia · 6 centers

• Federal State Autonomous Educational Institution of Higher Education "N.I. Pirogov Russian — Moscow
• Federal State Autonomous Educational Institution of Higher Education "N.I. Pirogov Russian — Moscow
• Federal State Autonomous Institution "National Medical Research Center for Children's Heal — Moscow
• Federal State Budgetary Educational Institution of Higher Education "St. Petersburg State — Saint Petersburg
• Federal State Budgetary Institution "V. A. Almazov National Medical Research Center" of th — Saint Petersburg
• State Autonomous Healthcare Institution of the Sverdlovsk Region "Regional Children's Clin — Yekaterinburg

Belarus · 1 center

• 1. State Institution Republican Scientific and Practical Center "Mother and Child" — Minsk

Identifiers

NCT: NCT05747261 · ANB-004-1

Primary sources (government registries)

• ClinicalTrials.gov (US NIH)

View this study on ClinicalTrials.gov ↗