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Recruiting NCT03080129

Microbiome and Sarcopenia in Patients With Liver Cirrhosis

No phase Interventional Sarcopenia Liver Cirrhosis

For patients and families

In plain language

An automatic summary of structured registry data. It is an orientation aid, not a substitute for the official protocol or a physician assessment.

What is being studied
The protocol lists: Fresubin energy.
Who it may be relevant to
Registry conditions: Sarcopenia, Liver Cirrhosis. Basic parameters: 18 years — 100 years · All.
What needs checking
Age, condition and sex are only basic indicators. Prior treatment, laboratory values and other mandatory requirements appear in the eligibility criteria below.
Where it takes place
Austria
Next step
Save the trial, show it to the treating physician, and confirm current recruitment with the study center. Costs, documents and travel →
Official title

Microbiome and Sarcopenia in Patients With Liver Cirrhosis: A Prospective Controlled Cohort Study

Overview

Protein-energy malnutrition (PEM) occurs in 65-90% of patients with liver cirrhosis. Severity of malnutrition correlates with progression of liver disease and leads to sarcopenia in 30-70% of cirrhotic patients. Malnutrition and sarcopenia are associated with an increased risk of complications and mortality. In cirrhosis the gut microbiome is altered leading to increased gut permeability, bacterial translocation and inflammation. Since the microbiome is involved in nutrient uptake and metabolism, it is hypothesized that microbiome alterations contribute to sarcopenia. A prospective controlled cohort study to investigate the interrelation of microbiome changes and sarcopenia in cirrhosis will be conducted. Furthermore the effect of nutritional interventions on the microbiome in cirrhosis will be studied. From this study information on how the gut microbiome composition and sarcopenia are associated in cirrhosis and if modulation of the gut microbiome by nutritional interventions is feasible will be collected.

Detailed description

Scientific background

Protein-energy malnutrition (PEM) occurs in 65-90% of patients with chronic liver disease. PEM is caused by various factors including poor dietary intake, loss of appetite, decreased hepatic protein synthesis, malabsorption and hypermetabolism. It is associated with an increased risk of complications including ascites, hepatic encephalopathy, variceal bleeding, hepatorenal syndrome and mortality. There is a direct relation between the progression of the liver disease and the severity of malnutrition.

Malnutrition and sarcopenia in liver cirrhosis patients

PEM leads to sarcopenia as a common, but frequently overlooked, complication. Sarcopenia is defined as a decrease in muscle mass two standard deviations below the healthy young adult mean. Sarcopenia is associated with aging, chronic diseases and malignancy. To determine the severity of muscle wasting, computed tomography scan (CT) or magnetic resonance imaging (MRI) are an objective and reproducible technique. Sarcopenia negatively impacts on survival, correlates with the risk of infections, increases surgical risk and leads to a poor quality of life. Besides PEM also inflammation is of importance in the development of sarcopenia.

Diversity in the microbiome in patients with liver cirrhosis and association with sarcopenia.

The gut microbiome of liver cirrhosis patients is altered compared to healthy individuals. Dysbiosis leads to an increased gut permeability, bacterial translocation and inflammation. This contributes to fibrogenesis and may also be related to hepatocarcinogenesis. Hence, new treatment approaches in cirrhosis focus on changing the microbial landscape.

Modulation the gut microbiome may also be a strategy to reverse sarcopenia by reducing systematic inflammation.

Hypothesis and aims

There is an association between gut microbiome composition, gut permeability and the existence of sarcopenia in cirrhotic patients.

Primary hypothesis: Diversity of the gut microbiome is reduced in liver cirrhosis patients with sarcopenia compared to those without sarcopenia or healthy controls.

Secondary hypotheses: There is an association between gut microbiome composition, biomarker of gut permeability and bacterial translocation with the presence of sarcopenia in cirrhosis. Oral nutrition supplements (ONS) can influence the composition of the gut microbiome, gut permeability, bacterial translocation and inflammation. Sarcopenia can be diagnosed from patients portraits.

Aims: to investigate:

* the composition of the gut microbiome * biomarkers of gut permeability, bacterial translocation and inflammation * the incidence and severity of sarcopenia * the impact of oral nutrition supplements (ONS) on the gut microbiome * whether artificial intelligence can be used to diagnose sarcopenia from face portraits.

Interventions

  • Dietary supplement Fresubin energy
    dietary protein energy supplement

Primary outcome measures

  • Alpha diversity [Time frame: day 1]
Secondary outcome measures (12)
  • Zonulin [Time frame: day 1]
  • diamino-oxidase [Time frame: day 1, day 7]
  • Calprotectin [Time frame: day 1]
  • Gut permeability [Time frame: change between day 1 and day 7]
  • taxonomic composition of the microbiome [Time frame: day 1]
  • taxonomic composition of the microbiome [Time frame: change between day 1 and day 7]
  • lipopolysaccharide [Time frame: day 1]
  • sCD14 [Time frame: day 1]
  • lipopolysaccharide binding protein [Time frame: day 1]
  • bacterial DNA [Time frame: day 1]
  • bacterial translocation [Time frame: change between day 1 and day 7]
  • cytokine panel [Time frame: day 1]

Eligibility criteria

Inclusion criteria

  • Hospitalized patients for any reason with clinical/radiological/histological diagnosis of cirrhosis
  • Age >18y
  • Informed consent
  • CT/MRI scan within +/-14 days of the baseline study visit

Exclusion criteria

  • Hepatic encephalopathy > grade 2 and or other cognitive disorder not allowing informed consent
  • advanced hepatocellular carcinoma
  • Any other condition or circumstance, which, in the opinion of the investigator, would affect the patient's ability to participate in the protocol

Criteria are shown verbatim from the registry (in English). Final eligibility is always assessed by the study center.

Healthy volunteers: Yes

Study design

Allocation
Non-randomized
Model
Parallel assignment
Masking
Single blind
Primary purpose
Basic science

Study locations

Austria · 1 center
  • Medical University Graz — Graz

Publications

  • Haller R, Feldbacher N, Furst S, Woltsche J, Gulden L, Schwarzl J, Traub J, Madl T, Habisch H, Horvath A, Stadlbauer V. Clinical and Mechanistic Association Between Intestinal Permeability and the Gut Microbiome in Cirrhosis: Role of Phascolarctobacterium. United European Gastroenterol J. 2026 Jul;14(6):e70262. doi: 10.1002/ueg2.70262. PMID 42438428
  • Woltsche J, Pacher-Deutsch C, Furst S, Gulden L, Schwarzl J, Feldbacher N, Nepel M, Celcer L, Hasl N, Rieper V, Stadlbauer V, Horvath A. Microbial Dysbiosis in the Urinary Microbiome of Patients With Cirrhosis. Am J Gastroenterol. 2026 Jan 1;121(1):262-267. doi: 10.14309/ajg.0000000000003634. Epub 2025 Jul 7. PMID 40622440

Identifiers

NCT: NCT03080129 · SAR

Primary sources (government registries)

View this study on ClinicalTrials.gov ↗